C57BL/6J-Tg(RP22-455H18)TG1Rdc/H

Status	Available to order
EMMA ID	EM:15944
Citation information	RRID:IMSR_EM:15944 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6J-Tg(RP22-455H18)TG1Rdc/H
Alternative name	C57BL/6J-Tg(RP22-455H18)TG1Rdc/H
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(RP22-455H18)TG1Rdc
Gene/Transgene symbol	Tg(RP22-455H18)TG1Rdc

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	In C57BL/6J mice, descended from the colony established at the Jackson Laboratory, we found that a spontaneous in-frame 5 exon deletion in Nnt was linked to glucose intolerance and reduced insulin secretion. We rescued the Nnt deletion in C57BL/6J mice by transgenic expression of the entire Nnt gene sequence contained within a bacterial artificial chromosome (BAC). This BAC transgenic line was prepared using BAC RP22-455H18 derived from a 129Sv mouse BAC library obtained from CHORI. We obtained two independent transgenic lines (B6-TG1 and B6-TG2) and in both lines expression of wildtype Nnt on the C57BL/6J background induced improvements in glucose tolerance and insulin secretion at 12 and 16 weeks of age following glucose challenge.
Phenotypic information	The mutation rescues impaired insulin secretion and glucose-intolerant phenotpe in C57BL/6J mice.
Breeding history	Generated on C57BL/6J background
References	Deletion of nicotinamide nucleotide transhydrogenase: a new quantitive trait locus accounting for glucose intolerance in C57BL/6J mice.;Freeman Helen C, Hugill Alison, Dear Neil T, Ashcroft Frances M, Cox Roger D, ;2006;Diabetes;55;2153-6; 16804088
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	not known
Immunocompromised	not known

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Literature references

Deletion of nicotinamide nucleotide transhydrogenase: a new quantitive trait locus accounting for glucose intolerance in C57BL/6J mice.;Freeman Helen C, Hugill Alison, Dear Neil T, Ashcroft Frances M, Cox Roger D, ;2006;Diabetes;55;2153-6; 16804088

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