C3H.B6-Pkd1l1^Rks/H

Status	Available to order
EMMA ID	EM:15945
Citation information	RRID:IMSR_EM:15945 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C3H.B6-Pkd1l1^Rks/H
Alternative name	C3H.B6-Pkd1l1/H
Strain type	Induced Mutant Strains
Allele/Transgene symbol	Pkd1l1^rks
Gene/Transgene symbol	Pkd1l1

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	RKS is an ENU derived mutant exhibiting perturbations of the left-right axis and carried a point mutation in Pkd1l1. A SNP based whole genome scan narrowed down the region of interest in RKS to a 14 Mega bases on Chromosome 11. This line will be used to carry out a complementation test with RKS. This BAC trangenic contains the Pkd1l1 locus, mutated in rks and will allow genetic rescue.
Phenotypic information	Homozygous: Homozygous mice display left-right abnormalities including pulmonary isomerism, stomach situs and gross cardiac situs. Heterozygous: Indistiguishable from wild-type littermates
Breeding history	Original mutant (CBMLC/81.2e male) was the offspring of a C57BL/6J male that received ENU mated to a C3H/HeH female.
References	Pkd1l1 establishes left-right asymmetry and physically interacts with Pkd2.;Field Sarah, Riley Kerry-Lyn, Grimes Daniel T, Hilton Helen, Simon Michelle, Powles-Glover Nicola, Siggers Pam, Bogani Debora, Greenfield Andy, Norris Dominic P, ;2011;Development (Cambridge, England);138;1131-42; 21307093
Homozygous fertile	not known
Homozygous viable	no
Homozygous matings required	not known
Immunocompromised	not known

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Literature references

Pkd1l1 establishes left-right asymmetry and physically interacts with Pkd2.;Field Sarah, Riley Kerry-Lyn, Grimes Daniel T, Hilton Helen, Simon Michelle, Powles-Glover Nicola, Siggers Pam, Bogani Debora, Greenfield Andy, Norris Dominic P, ;2011;Development (Cambridge, England);138;1131-42; 21307093

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Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
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C3H.B6-Pkd1l1Rks/H