IMPC phenotypes (gene matching)
- increased spleen weight / IMPC
B6JRcc.129(Cg)-Ccl17tm1.1(HBEGF)Irfo/Ieg
| Status | Available to order |
| EMMA ID | EM:15955 |
| Citation information | RRID:IMSR_EM:15955 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6JRcc.129(Cg)-Ccl17tm1.1(HBEGF)Irfo/Ieg |
| Alternative name | B6.129-CCL17tm1(DTR)Irfo/J |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Ccl17tm1.1(HBEGF)Irfo |
| Gene/Transgene symbol | Ccl17 |
Information from provider
| Provider | Irmgard Förster |
| Provider affiliation | LIMES/Immunology and Environment, University of Bonn |
| Genetic information | The simian diphtheria toxin receptor (DTR, now heparin binding EGF-like growth factor or HBEGF) is expressed under the control of the Ccl17 promoter. Homozygous mice are deficient for CCL17. For generation of CCL17DTR mice expressing the simian DTR, the DTR cDNA and a neomycin cassette flanked by flippase recognition target (FRT)-recombination sites were inserted in the second exon of the mouse Ccl17 gene. Homologously recombined 129P2 (E14.1) embryonic stem cell clones were detected by Southern blot and the neomycin-resistance cassette was removed from the targeted Ccl17 locus by flippase (FLP) recombinase expression. |
| Phenotypic information | Homozygous:Expression of the diphtheria toxin receptor under the control of the Ccl17 promoter enables depletion of CCL17-expressing cells in vivo by injection of diphtheria toxin. In mice, CCL17 is expressed by a subpopulation of dendritic cells and a small fraction of hippocampal neurons. Homozygous mice (DTR/DTR) are deficient for CCL17, are viable, fertile, normal in size and weight, and show no physical or behavioral abnormalities.Heterozygous:The same like homozygous, but with expression of CCL17 from the wild-type allele. |
| Breeding history | After germline transmission, mice were backcrossed to the C57BL/6JRcc background for more than 8 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | homozygous C57BL/6J males |
Disease and phenotype information
Literature references
- Ablation of CCL17-positive hippocampal neurons induces inflammation-dependent epilepsy.;Eberhard Judith, Henning Lukas, Fülle Lorenz, Knöpper Konrad, Böhringer Jana, Graelmann Frederike J, Hänschke Lea, Kenzler Julia, Brosseron Frederic, Heneka Michael T, Domingos Ana I, Eyerich Stefanie, Lochner Matthias, Weighardt Heike, Bedner Peter, Steinhäuser Christian, Förster Irmgard, ;2025;Epilepsia;66;554-568; 39607395