CD1;129-Ibsp^tm1Jeau/Orl

Status	Available to order
EMMA ID	EM:15962
Citation information	RRID:IMSR_EM:15962 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	CD1;129-Ibsp^tm1Jeau/Orl
Alternative name	BSP knockout, Ibsp-/-
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Ibsp^tm1Jeau
Gene/Transgene symbol	Ibsp

Information from provider

Provider	Luc Malaval
Provider affiliation	U1059-SAINBIOSE, INSERM
Additional owner	Dr Jane E Aubin, Professor Emerita, University of Toronto. Canada.
Genetic information	In this line the Ibsp gene, coding for bone sialoprotein, was knocked-out through homologous recombination (Malaval L et al., J Exp Med., 2008, 205(5):1145-1153).
Phenotypic information	Homozygous: Ibsp-/- mice are viable and breed normally, but their weight and size are lower than wild-type mice. Malocclusion occurs sporadically in homozygous mutant mice. Bone matrix is undermineralized. Ibsp-/- mice display thinner cortical bones than wild-type, but greater trabecular bone volume at 4 months with very low bone formation rate and reduced resorption. The mutation also affects the local environment of the bone tissue, in particular hematopoiesis and vascularization (Bouleftour et al., Matrix Biol., 2016, 52–54, 60–77). Heterozygous: The phenotype of heterozygous mice is indistinguable from that of wild-type mice of same genetic background.
Breeding history	Two Ibsp mouse cDNA clones were used to screen a 129Sv/J mouse genomic λDASH2 phage library; 2 overlapping genomic clones, spanning ~19 kb of sequence and the entire Ibsp locus, were isolated and sequenced. A short 5’ arm of Ibsp homology on a 600-bp Sac1 fragment downstream of exon 1 was cloned upstream of the PGKneo cassette in the pPNT positive-negative selection vector. A long 3’ arm of Ibsp homology on a 6-kb Kpn fragment was then cloned downstream of the PGKneo cassette, giving the final targeting vector. Homologous recombination resulted in the deletion of exons 2–3 and insertion of the PGKneo cassette in their place. R1 (129Sv) mouse embryonic stem cells (passage 8) were provided by A. Nagy (Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada). R1 embryonic stem cells were electroporated with the NotI-linearized targeting vector and were selected by using 200 μg/ml of active G418 geneticin (Invitrogen) and 2 μM ganciclovir (Syntex, Inc.). DNA from selected clones was digested with HindIII and analyzed by Southern blot hybridization using a HindIII/Xba probe. Positive clones were used with CD1 embryos to make chimeric mice, among which a male transmitted the mutation through the germ line after crossing with albino CD1 outbred females. Offspring were maintained on a 129Sv/CD-1 background.
References	Absence of bone sialoprotein (BSP) alters profoundly hematopoiesis and upregulates osteopontin.;Granito Renata Neves, Bouleftour Wafa, Sabido Odile, Lescale Chloé, Thomas Mireille, Aubin Jane E, Goodhardt Michèle, Vico Laurence, Malaval Luc, ;2015;Journal of cellular physiology;230;1342-51; 25502698 Absence of bone sialoprotein (BSP) impairs cortical defect repair in mouse long bone.;Malaval Luc, Monfoulet Laurent, Fabre Thierry, Pothuaud Laurent, Bareille Reine, Miraux Sylvain, Thiaudiere Eric, Raffard Gerard, Franconi Jean-Michel, Lafage-Proust Marie-Hélène, Aubin Jane E, Vico Laurence, Amédée Joëlle, ;2009;Bone;45;853-61; 19524706 Blocking the expression of both bone sialoprotein (BSP) and osteopontin (OPN) impairs the anabolic action of PTH in mouse calvaria bone.;Bouleftour Wafa, Bouet Guenaelle, Granito Renata Neves, Thomas Mireille, Linossier Marie-Thérèse, Vanden-Bossche Arnaud, Aubin Jane E, Lafage-Proust Marie-Hélène, Vico Laurence, Malaval Luc, ;2015;Journal of cellular physiology;230;568-77; 25160656 Mice lacking bone sialoprotein (BSP) lose bone after ovariectomy and display skeletal site-specific response to intermittent PTH treatment.;Wade-Gueye Ndéye Marième, Boudiffa Maya, Laroche Norbert, Vanden-Bossche Arnaud, Fournier Carole, Aubin Jane E, Vico Laurence, Lafage-Proust Marie-Hélène, Malaval Luc, ;2010;Endocrinology;151;5103-13; 20844009 Skeletal development of mice lacking bone sialoprotein (BSP)--impairment of long bone growth and progressive establishment of high trabecular bone mass.;Bouleftour Wafa, Boudiffa Maya, Wade-Gueye Ndeye Marième, Bouët Guénaëlle, Cardelli Marco, Laroche Norbert, Vanden-Bossche Arnaud, Thomas Mireille, Bonnelye Edith, Aubin Jane E, Vico Laurence, Lafage-Proust Marie Hélène, Malaval Luc, ;2014;PloS one;9;e95144; 24816232 The role of the SIBLING, Bone Sialoprotein in skeletal biology - Contribution of mouse experimental genetics.;Bouleftour Wafa, Juignet Laura, Bouet Guenaelle, Granito Renata Neves, Vanden-Bossche Arnaud, Laroche Norbert, Aubin Jane E, Lafage-Proust Marie-Hélène, Vico Laurence, Malaval Luc, ;2016;Matrix biology : journal of the International Society for Matrix Biology;-5254;60-77; 26763578 OPN, BSP, and Bone Quality-Structural, Biochemical, and Biomechanical Assessment in OPN^-/-, BSP^-/-, and DKO Mice.;Malaval Luc, Follet Hélène, Farlay Delphine, Gineyts Evelyne, Rizzo Sebastien, Thomas Charlene, Maalouf Mathieu, Normand Myriam, Burt-Pichat Brigitte, Bouleftour Wafa, Vanden-Boscche Arnaud, Laroche Norbert, Vico Laurence, ;2024;Calcified tissue international;115;63-77; 38733411 Bone sialoprotein plays a functional role in bone formation and osteoclastogenesis.;Malaval Luc, Wade-Guéye Ndéyé Marième, Boudiffa Maya, Fei Jia, Zirngibl Ralph, Chen Frieda, Laroche Norbert, Roux Jean-Paul, Burt-Pichat Brigitte, Duboeuf François, Boivin Georges, Jurdic Pierre, Lafage-Proust Marie-Hélène, Amédée Joëlle, Vico Laurence, Rossant Janet, Aubin Jane E, ;2008;The Journal of experimental medicine;205;1145-53; 18458111 Absence of bone sialoprotein (BSP) impairs primary bone formation and resorption: the marrow ablation model under PTH challenge.;Wade-Gueye Ndéye Marième, Boudiffa Maya, Vanden-Bossche Arnaud, Laroche Norbert, Aubin Jane E, Vico Laurence, Lafage-Proust Marie-Hélène, Malaval Luc, ;2012;Bone;50;1064-73; 22586700 Bone Shaft Revascularization After Marrow Ablation Is Dramatically Accelerated in BSP-/- Mice, Along With Faster Hematopoietic Recolonization.;Bouleftour Wafa, Granito Renata Neves, Vanden-Bossche Arnaud, Sabido Odile, Roche Bernard, Thomas Mireille, Linossier Marie Thérèse, Aubin Jane E, Lafage-Proust Marie-Hélène, Vico Laurence, Malaval Luc, ;2017;Journal of cellular physiology;232;2528-2537; 27704558
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	not known

Information from EMMA

Archiving centre	CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France
Animals used for archiving	homozygous CD1 males

Disease and phenotype information

MGI phenotypes (gene matching)

decreased bone mineral density / MGI
large anterior fontanelle / MGI
malocclusion / MGI
abnormal trabecular bone morphology / MGI
decreased compact bone thickness / MGI
abnormal mandible morphology / MGI
decreased body weight / MGI
decreased body size / MGI
abnormal molar morphology / MGI
abnormal bone mineralization / MGI
abnormal bone remodeling / MGI
short femur / MGI
delayed intramembranous bone ossification / MGI
abnormal periodontal ligament morphology / MGI
abnormal osteoclast morphology / MGI
increased osteoclast cell number / MGI
decreased osteoclast cell number / MGI
abnormal osteoblast morphology / MGI
decreased osteoblast cell number / MGI
increased bone resorption / MGI
abnormal incisor morphology / MGI
craniofacial phenotype / MGI
skeleton phenotype / MGI
decreased trabecular bone thickness / MGI
delayed cranial suture closure / MGI
increased bone trabecula number / MGI
increased trabecular bone volume / MGI
abnormal osteoid morphology / MGI
increased osteoid thickness / MGI
abnormal tooth root development / MGI
decreased bone trabecular spacing / MGI
abnormal periodontium morphology / MGI
wide coronal suture / MGI
wide lambdoid suture / MGI
delayed fontanelle closure / MGI
abnormal cementum mineralization / MGI
alveolar process atrophy / MGI
abnormal molar root morphology / MGI
abnormal junctional epithelium morphology / MGI
abnormal tooth root resorption / MGI
misaligned incisors / MGI
abnormal acellular cementum morphology / MGI

Literature references

Absence of bone sialoprotein (BSP) alters profoundly hematopoiesis and upregulates osteopontin.;Granito Renata Neves, Bouleftour Wafa, Sabido Odile, Lescale Chloé, Thomas Mireille, Aubin Jane E, Goodhardt Michèle, Vico Laurence, Malaval Luc, ;2015;Journal of cellular physiology;230;1342-51; 25502698
Absence of bone sialoprotein (BSP) impairs cortical defect repair in mouse long bone.;Malaval Luc, Monfoulet Laurent, Fabre Thierry, Pothuaud Laurent, Bareille Reine, Miraux Sylvain, Thiaudiere Eric, Raffard Gerard, Franconi Jean-Michel, Lafage-Proust Marie-Hélène, Aubin Jane E, Vico Laurence, Amédée Joëlle, ;2009;Bone;45;853-61; 19524706
Blocking the expression of both bone sialoprotein (BSP) and osteopontin (OPN) impairs the anabolic action of PTH in mouse calvaria bone.;Bouleftour Wafa, Bouet Guenaelle, Granito Renata Neves, Thomas Mireille, Linossier Marie-Thérèse, Vanden-Bossche Arnaud, Aubin Jane E, Lafage-Proust Marie-Hélène, Vico Laurence, Malaval Luc, ;2015;Journal of cellular physiology;230;568-77; 25160656
Mice lacking bone sialoprotein (BSP) lose bone after ovariectomy and display skeletal site-specific response to intermittent PTH treatment.;Wade-Gueye Ndéye Marième, Boudiffa Maya, Laroche Norbert, Vanden-Bossche Arnaud, Fournier Carole, Aubin Jane E, Vico Laurence, Lafage-Proust Marie-Hélène, Malaval Luc, ;2010;Endocrinology;151;5103-13; 20844009
Skeletal development of mice lacking bone sialoprotein (BSP)--impairment of long bone growth and progressive establishment of high trabecular bone mass.;Bouleftour Wafa, Boudiffa Maya, Wade-Gueye Ndeye Marième, Bouët Guénaëlle, Cardelli Marco, Laroche Norbert, Vanden-Bossche Arnaud, Thomas Mireille, Bonnelye Edith, Aubin Jane E, Vico Laurence, Lafage-Proust Marie Hélène, Malaval Luc, ;2014;PloS one;9;e95144; 24816232
The role of the SIBLING, Bone Sialoprotein in skeletal biology - Contribution of mouse experimental genetics.;Bouleftour Wafa, Juignet Laura, Bouet Guenaelle, Granito Renata Neves, Vanden-Bossche Arnaud, Laroche Norbert, Aubin Jane E, Lafage-Proust Marie-Hélène, Vico Laurence, Malaval Luc, ;2016;Matrix biology : journal of the International Society for Matrix Biology;-5254;60-77; 26763578
OPN, BSP, and Bone Quality-Structural, Biochemical, and Biomechanical Assessment in OPN^-/-, BSP^-/-, and DKO Mice.;Malaval Luc, Follet Hélène, Farlay Delphine, Gineyts Evelyne, Rizzo Sebastien, Thomas Charlene, Maalouf Mathieu, Normand Myriam, Burt-Pichat Brigitte, Bouleftour Wafa, Vanden-Boscche Arnaud, Laroche Norbert, Vico Laurence, ;2024;Calcified tissue international;115;63-77; 38733411
Bone sialoprotein plays a functional role in bone formation and osteoclastogenesis.;Malaval Luc, Wade-Guéye Ndéyé Marième, Boudiffa Maya, Fei Jia, Zirngibl Ralph, Chen Frieda, Laroche Norbert, Roux Jean-Paul, Burt-Pichat Brigitte, Duboeuf François, Boivin Georges, Jurdic Pierre, Lafage-Proust Marie-Hélène, Amédée Joëlle, Vico Laurence, Rossant Janet, Aubin Jane E, ;2008;The Journal of experimental medicine;205;1145-53; 18458111
Absence of bone sialoprotein (BSP) impairs primary bone formation and resorption: the marrow ablation model under PTH challenge.;Wade-Gueye Ndéye Marième, Boudiffa Maya, Vanden-Bossche Arnaud, Laroche Norbert, Aubin Jane E, Vico Laurence, Lafage-Proust Marie-Hélène, Malaval Luc, ;2012;Bone;50;1064-73; 22586700
Bone Shaft Revascularization After Marrow Ablation Is Dramatically Accelerated in BSP-/- Mice, Along With Faster Hematopoietic Recolonization.;Bouleftour Wafa, Granito Renata Neves, Vanden-Bossche Arnaud, Sabido Odile, Roche Bernard, Thomas Mireille, Linossier Marie Thérèse, Aubin Jane E, Lafage-Proust Marie-Hélène, Vico Laurence, Malaval Luc, ;2017;Journal of cellular physiology;232;2528-2537; 27704558

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CD1;129-Ibsptm1Jeau/Orl