129-Itgb1bp2tm1Tron/Cnrm
| Status | Available to order |
| EMMA ID | EM:15967 |
| Citation information | RRID:IMSR_EM:15967 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | 129-Itgb1bp2tm1Tron/Cnrm |
| Alternative name | Itgb1bp2 -/- (melusin -/-) 129SV |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Itgb1bp2tm1Tron |
| Gene/Transgene symbol | Itgb1bp2 |
Information from provider
| Provider | Mara Brancaccio |
| Provider affiliation | University of Torino |
| Genetic information | Using the murine cDNA probe, we isolated a genomic fragment of 14.8 kb encompassing the Itgb1bp2 (melusin) first exon, which contains the ATG start codon, and three other exons. A PstI fragment containing exons 1-4 was replaced with a cassette containing IRES sequences linked to the lacZ gene followed by the neomycin-resistance gene driven by a PGK promoter. |
| Phenotypic information | Homozygous:Itgb1bp2-/- mice showed normal cardiac structure and function in physiological conditions, but when subjected to pressure overload, a condition that induces a hypertrophic response in wild-type controls, they developed an abnormal cardiac remodeling that evolved into dilated cardiomyopathy and contractile dysfunction (Brancaccio M et al., Nat Med 9, 68–75, 2003).Heterozygous:Itgb1bp2 is on the X chromosome. Males are hemizygous and do not show phenotypes. |
| Breeding history | ES recombinant clones (129Sv) have been injected into C57BL/6 blastocysts to generate chimeras. Chimeras have been mated with 129Sv and offspring of different chimeras were mated together. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- Melusin, a muscle-specific integrin beta1-interacting protein, is required to prevent cardiac failure in response to chronic pressure overload.;Brancaccio Mara, Fratta Luigi, Notte Antonella, Hirsch Emilio, Poulet Roberta, Guazzone Simona, De Acetis Marika, Vecchione Carmine, Marino Gennaro, Altruda Fiorella, Silengo Lorenzo, Tarone Guido, Lembo Giuseppe, ;2003;Nature medicine;9;68-75; 12496958
- An intrinsic mechanism of metabolic tuning promotes cardiac resilience to stress.;Sorge Matteo, Savoré Giulia, Gallo Andrea, Acquarone Davide, Sbroggiò Mauro, Velasco Silvia, Zamporlini Federica, Femminò Saveria, Moiso Enrico, Morciano Giampaolo, Balmas Elisa, Raimondi Andrea, Nattenberg Gabrielle, Stefania Rachele, Tacchetti Carlo, Rizzo Angela Maria, Corsetto Paola, Ghigo Alessandra, Turco Emilia, Altruda Fiorella, Silengo Lorenzo, Pinton Paolo, Raffaelli Nadia, Sniadecki Nathan J, Penna Claudia, Pagliaro Pasquale, Hirsch Emilio, Riganti Chiara, Tarone Guido, Bertero Alessandro, Brancaccio Mara, ;2024;EMBO molecular medicine;16;2450-2484; 39271959