Igf2-IRES-Dre-T2A-Katushka
| Status | Available to order |
| EMMA ID | EM:15985 |
| Citation information | RRID:IMSR_EM:15985 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | Igf2-IRES-Dre-T2A-Katushka |
| Alternative name | Igf2-IRES-Dre-T2A-Katushka |
| Strain type | Targeted Mutant Strains |
| Allele/Transgene symbol | Igf2 |
| Gene/Transgene symbol | Igf2 |
Information from provider
| Provider | Sophie Storrar |
| Provider affiliation | UK Health Security Agency |
| Genetic information | These Igf2-IRES-Dre-T2A-Katushka mutant mice express both Dre recombinase and Katushka under the control of the endogenous Igf2 promoter. The mutation was introduced by AAV-transduction and electroporation with assembled RNPs of C57BL/6J embryos (CRISPR-READI). The model was modified by HDR using the Cas9-3xNLS protein, following the CRISPR-READI methodology, similar to that detailed in the paper by Chen et al (PMID: 31242412). |
| Phenotypic information | Homozygous:Mice that are homozygous for the mutation are not predicted to display any gross physical or behavioral abnormalities.Heterozygous:Mice that are heterozygous for the mutation are viable and fertile. Mice produced to date have not shown any gross physical or behavioral abnormalities. |
| Breeding history | C57BL/6J embryos were utilized to generate the initial mutation via CRISPR-READI. These Igf2-IRES-Dre-T2A-Katushka mutant mice have been backcrossed for at least 1 generation to C57BL/6J mice. These mice are heterozygous for the mutation. |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |