B6(Cg)-Sp8tm-Maros/Cnbc
| Status | Available to order |
| EMMA ID | EM:15987 |
| Citation information | RRID:IMSR_EM:15987 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6(Cg)-Sp8tm-Maros/Cnbc |
| Alternative name | Sp8:3xFlagtmMaros |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Sp8 |
| Gene/Transgene symbol | Sp8 |
Information from provider
| Provider | Marian Ros |
| Provider affiliation | Señalizacion Celular y Molecular, CSIC-IBBTEC |
| Genetic information | Three copies of the FLAG epitope were inserted in frame at the C-terminal domain of Sp8. The knocking-in was performed by Cyagen Biosciences Inc. using ES cells. The FLAG epitope is recognized by specific antibodies (immunoflurescence and Western blotting) in samples from the Sp8:3xFlag strain. |
| Phenotypic information | Homozygous:Wild-type phenotypeHeterozygous:Wild-type phenotype |
| Breeding history | The strain has been maintained in the C57BL/6 background since 2018. Both in hetero- and homozygous state. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous C57BL/6JOlaHsd males |