129.B6-Sod1em1(SOD1)Emcf/H
| Status | Available to order |
| EMMA ID | EM:16005 |
| Citation information | RRID:IMSR_EM:16005 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | 129.B6-Sod1em1(SOD1)Emcf/H |
| Alternative name | 129.B6-Sod1 |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Sod1em1(SOD1)Emcf |
| Gene/Transgene symbol | Sod1 |
Information from provider
| Provider | MRC, Medical Research Council |
| Provider affiliation | Mary Lyon Centre at MRC Harwell |
| Genetic information | 129.B6-Sod1em1(SOD1)Emcf/H knock-in mice carry the human version of the Cu,Zn-superoxide dismutase-1 gene (SOD1) gene with a missense alanine to valine mutation at codon 4 (A4V; allele designated Sod1em1(SOD1)Emcf, MGI:6856511). The humanized SOD1-A4V gene replaces the mouse Sod1 gene at the endogenous locus. The humanized region begins at the first codon in exon 1 of the SOD1 gene, and continues through to the end of the human SOD1 5’ UTR. Exons 4, 5 and the 5’ UTR of the humanized SOD1 gene have been floxed and duplicated to allow conditional expression of a second copy of exons 4 and 5. These mice were generated using endonuclease mediated homologous recombination. Wild-type Cas9 protein purchased from Sigma (now Merck). The Cas9 and guides were delivered as RNPs so no Cas9 or guide sequences were integrated into the genome. |
| Phenotypic information | Homozygous:No phenotype data availableHeterozygous:No phenotype data available |
| Breeding history | Congenic on 129 |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Amyotrophic lateral sclerosis / Orphanet_803