C57BL/6N-Glrbtm1Ics/Ics

Status

Available to order

EMMA IDEM:16066
Citation informationRRID:IMSR_EM:16066 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

International strain nameC57BL/6N-Glrbtm1Ics/Ics
Alternative nameGlrbtm1Ics/Ics
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolGlrbtm1Ics
Gene/Transgene symbolGlrb

Information from provider

ProviderChristian Specht
Provider affiliationInserm U1195 / Université Paris-Saclay, Unité DHNS – Maladies et Hormones du Système Nerveux
Genetic informationThe targeting cassette inserted the coding sequence of the mutated coral-derived green-to-red photoconvertible fluorescent reporter protein (mEos4b) after the signal peptide leader sequence. A self-excising neomycin selection cassette is absent from the final allele.
Phenotypic informationHomozygous:
N/A

Heterozygous:
N/A
Breeding historyPure C57BL/6N genetic background. Current background: C57BL/6NCrl 100%
References
  • Identification of a stereotypic molecular arrangement of endogenous glycine receptors at spinal cord synapses.;Maynard Stephanie A, Rostaing Philippe, Schaefer Natascha, Gemin Olivier, Candat Adrien, Dumoulin Andréa, Villmann Carmen, Triller Antoine, Specht Christian G, ;2021;eLife;10;; 34878402
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

Disease and phenotype information

IMPC phenotypes (gene matching)
  • increased spleen weight / IMPC
  • increased neutrophil cell number / IMPC
  • increased circulating aspartate transaminase level / IMPC
  • decreased circulating serum albumin level / IMPC
  • increased heart weight / IMPC
  • decreased freezing behavior / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • increased circulating alanine transaminase level / IMPC
  • decreased circulating alkaline phosphatase level / IMPC
MGI phenotypes (gene matching)
  • fragile skeleton / MGI
  • impaired fertilization / MGI
  • enlarged adrenal glands / MGI
  • muscle spasm / MGI
  • tremors / MGI
  • abnormal Purkinje cell morphology / MGI
  • abnormal spinal cord morphology / MGI
  • abnormal dorsal root ganglion morphology / MGI
  • abnormal spinal nerve morphology / MGI
  • decreased body size / MGI
  • abnormal maternal nurturing / MGI
  • abnormal gait / MGI
  • short stride length / MGI
  • increased startle reflex / MGI
  • abnormal posture / MGI
  • limb grasping / MGI
  • impaired swimming / MGI
  • impaired righting response / MGI
  • edema / MGI
  • hemorrhage / MGI
  • reduced male fertility / MGI
  • abnormal motor capabilities/coordination/movement / MGI
  • muscle hypertonia / MGI
  • abnormal intervertebral disk morphology / MGI
  • impaired acrosome reaction / MGI
  • abnormal vertebral column morphology / MGI
  • abnormal spinal cord ventral horn morphology / MGI
  • hyperekplexia / MGI
  • abnormal adrenal cortex morphology / MGI
  • abnormal brain dura mater morphology / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • preweaning lethality, complete penetrance / MGI

Literature references

  • Identification of a stereotypic molecular arrangement of endogenous glycine receptors at spinal cord synapses.;Maynard Stephanie A, Rostaing Philippe, Schaefer Natascha, Gemin Olivier, Candat Adrien, Dumoulin Andréa, Villmann Carmen, Triller Antoine, Specht Christian G, ;2021;eLife;10;; 34878402

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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