C57BL/6N-Ptchd1^{tm1a(KOMP)IcsOrl}/Ics

Status	Available to order
EMMA ID	EM:16070
Citation information	RRID:IMSR_EM:16070 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-Ptchd1^{tm1a(KOMP)IcsOrl}/Ics
Alternative name	Ptchd1tm1a(KOMP)IcsOrl/Ics
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Ptchd1^{tm1a(KOMP)IcsOrl}
Gene/Transgene symbol	Ptchd1

Information from provider

Provider	ICS, Institut Clinique de la Souris
Provider affiliation	ICS, Institut Clinique de la Souris
Genetic information	The KOMP vector PRPGS00100_C_H03 was inserted at position 155587519 of Chromosome X upstream of exon 2 (Build GRCm38). The cassette is composed of an FRT site followed by a lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, an SV40 polyA sequence, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 155587393. Exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knock-out mouse. If cre expression occurs without flp expression, a reporter knock-out mouse will be created.
Phenotypic information	Homozygous: Homozygous animals are subviable. Heterozygous: N/A
Breeding history	Pure C57BL/6N genetic background. Current background: C57BL/6NTac 100%
References	None available
Homozygous fertile	not known
Homozygous viable	no
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

Disease and phenotype information

IMPC phenotypes (gene matching)

hyperactivity / IMPC
abnormal auditory brainstem response / IMPC
increased aggression / IMPC
abnormal vocalization / IMPC
decreased bone mineral density / IMPC
decreased erythrocyte cell number / IMPC
abnormal contextual conditioning behavior / IMPC
increased circulating creatinine level / IMPC
increased exploration in new environment / IMPC
increased lean body mass / IMPC
abnormal spleen morphology / IMPC
increased vertical activity / IMPC
abnormal locomotor behavior / IMPC
abnormal cued conditioning behavior / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Right strain for your research?

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C57BL/6N-Ptchd1tm1a(KOMP)IcsOrl/Ics