C57BL/6N-Tmem176a/btm1.1Ics/Ics
| Status | Available to order |
| EMMA ID | EM:16076 |
| Citation information | RRID:IMSR_EM:16076 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6N-Tmem176a/btm1.1Ics/Ics |
| Alternative name | Tmem176btm2.1Ics/Ics |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Tmem176a/btm1.1Ics |
| Gene/Transgene symbol | Tmem176a/b |
Information from provider
| Provider | Cédric Louvet |
| Provider affiliation | Centre de Recherche en Transplantation et Immunologie (CRTI), Université de Nantes, Inserm UMR 1064, |
| Genetic information | A loxP site followed by a F3-HygroR-F3 cassette was inserted into Tmem176b-202 (GRCm39) intron 2. A FRT-NeoR-FRT cassette followed by a second loxP site was inserted into Tmem176a-201 intron 3. The two selection cassettes were removed by flp-mediated recombination using a C57BL/6N flpo deleter mouse line (MGI:5285396), which led to the conditional-ready allele for both Tmem176a and Tmem176b genes. |
| Phenotypic information | Homozygous:N/AHeterozygous:N/A |
| Breeding history | Pure C57BL/6N genetic background. Current background: C57BL/6N 0.0106%, C57BL/6NCrl 86.7188%, C57BL/6NTac 13.2706% |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France |
Literature references
- Dendritic Cells Require TMEM176A/B Ion Channels for Optimal MHC Class II Antigen Presentation to Naive CD4+ T Cells.;Lancien Melanie, Bienvenu Geraldine, Salle Sonia, Gueno Lucile, Feyeux Magalie, Merieau Emmanuel, Remy Severine, Even Amandine, Moreau Aurelie, Molle Alice, Fourgeux Cynthia, Coulon Flora, Beriou Gaelle, Bouchet-Delbos Laurence, Chiffoleau Elise, Kirstetter Peggy, Chan Susan, Kerfoot Steven M, Abdu Rahiman Saeed, De Simone Veronica, Matteoli Gianluca, Boncompain Gaelle, Perez Franck, Josien Regis, Poschmann Jeremie, Cuturi Maria Cristina, Louvet Cedric, ;2021;Journal of immunology (Baltimore, Md. : 1950);207;421-435; 34233909