C57BL/6N-Bcl2l1tm2.1Ics/Ics
| Status | Available to order |
| EMMA ID | EM:16127 |
| Citation information | RRID:IMSR_EM:16127 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6N-Bcl2l1tm2.1Ics/Ics |
| Alternative name | Bcl2l1tm2.1Ics/Ics |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Bcl2l1tm2.1Ics |
| Gene/Transgene symbol | Bcl2l1 |
Information from provider
| Provider | Ivan Mikaelian |
| Provider affiliation | Signalisation, métabolisme et progression tumorale, Centre de Recherche en Cancérologie de Lyon (CRCL) |
| Genetic information | A loxP site was inserted into intron 2 and 3x poly(A) signal sequence was inserted into exon 3 (Ensembl:Bcl2l1-201, GRCm39) immediately downstream of the stop codon, followed by an FRT site flanked neomycin resistance gene cassette and a second loxP site. Downstream, 298 bp of intron 2 and a modified exon 3 [where the sequence coding for the C-terminal transmembrane (TM) motif was replaced with sequence coding for the Acta1 mitochondrial targeting (MITO) signal] were inserted. The neo cassette was removed through flp-mediated recombination, leaving the endogenous exon 3 floxed. This allele expresses the wild-type protein and only after cre-mediated deletion of the endogenous exon 3 will it express the mitochondria-directed peptide (see EMMA strain EM:15923). |
| Phenotypic information | Homozygous:N/AHeterozygous:N/A |
| Breeding history | 100% C57BL/6N |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France |
Literature references
- Mitochondrial Bcl-xL promotes brain synaptogenesis by controlling non-lethal caspase activation.;Nguyen Trang Thi Minh, Gadet Rudy, Lanfranchi Marine, Lahaye Romane A, Yandiev Sozerko, Lohez Olivier, Mikaelian Ivan, Jabbour Lea, Rimokh Ruth, Courchet Julien, Saudou Frédéric, Popgeorgiev Nikolay, Gillet Germain, ;2023;iScience;26;106674; 37182099