B6N(Cg)-Slit2tm1.1Ics/Ics

Status

Available to order

EMMA IDEM:16157
Citation informationRRID:IMSR_EM:16157 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

International strain nameB6N(Cg)-Slit2tm1.1Ics/Ics
Alternative nameSlit2tm1.1Ics/Ics
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolSlit2tm1.1Ics
Gene/Transgene symbolSlit2

Information from provider

ProviderAlain Chedotal
Provider affiliationInstitut de la vision
Genetic informationA LoxP site was inserted into intron 7. A second LoxP site followed by an FRT flanked neomycin resistance were inserted into intron 8. The neo cassette was removed through subsequent flp-mediated recombination (MGI:2158498), leaving a conditional-ready allele with exon 8 (ENSMUSE00001269610; GRCm39, Slit2-208) floxed.
Phenotypic informationHomozygous:
N/A

Heterozygous:
N/A
Breeding history73.4% C57BL/6N; the line was backcrossed 2 times with C57BL/6N mice (C57BL/6N 73.43%, FVB/N 0.78%, 129/SvPas 25%, SJL/J 0.78%).
References
  • Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization.;Rama Nicolas, Dubrac Alexandre, Mathivet Thomas, Ní Chárthaigh Róisín-Ana, Genet Gael, Cristofaro Brunella, Pibouin-Fragner Laurence, Ma Le, Eichmann Anne, Chédotal Alain, ;2015;Nature medicine;21;483-91; 25894826
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

Disease and phenotype information

IMPC phenotypes (gene matching)
  • abnormal spleen morphology / IMPC
  • abnormal kidney morphology / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • abnormal placenta vasculature / IMPC
  • abnormal placenta morphology / IMPC
  • enlarged spleen / IMPC
  • enlarged kidney / IMPC
  • cataract / IMPC
  • hydrometra / IMPC
  • microphthalmia / IMPC
  • abnormal vitreous body morphology / IMPC

Literature references

  • Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization.;Rama Nicolas, Dubrac Alexandre, Mathivet Thomas, Ní Chárthaigh Róisín-Ana, Genet Gael, Cristofaro Brunella, Pibouin-Fragner Laurence, Ma Le, Eichmann Anne, Chédotal Alain, ;2015;Nature medicine;21;483-91; 25894826

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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