B6N(B6J)-Esr1^tm3.1Ics/Ics

Status	Available to order
EMMA ID	EM:16161
Citation information	RRID:IMSR_EM:16161 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6N(B6J)-Esr1^tm3.1Ics/Ics
Alternative name	Esr1tm3.1Ics/Ics
Strain type	Targeted Mutant Strains : Point mutation
Allele/Transgene symbol	Esr1^tm3.1Ics
Gene/Transgene symbol	Esr1

Information from provider

Provider	Jean Francois Amal
Provider affiliation	Institut des Maladies Métaboliques et Cardiovasculaires, INSERM/UPS U1297 – I2MC
Genetic information	Arginine 264 (CGA) in exon 5 was changed to alanine (GCA) and a loxP site flanked neomycin resistance gene cassette was inserted into intron 5. The neo cassette was removed through subsequent cre-mediated recombination. The mutation abrogates the rapid dilation of mesenteric arteries and the acceleration of endothelial repair of carotid actions of the encoded peptide without affecting other functions.
Phenotypic information	Homozygous: N/A Heterozygous: N/A
Breeding history	96.87% C57BL/6N; the line was backcrossed 2 times with C57BL/6NTac mice (C57BL/6J 3.12%, C57BL/6N 25%, C57BL/6NTac 71.8%).
References	Mutation of Arginine 264 on ERα (Estrogen Receptor Alpha) Selectively Abrogates the Rapid Signaling of Estradiol in the Endothelium Without Altering Fertility.;Adlanmerini Marine, Fébrissy Chanaelle, Zahreddine Rana, Vessières Emilie, Buscato Mélissa, Solinhac Romain, Favre Julie, Anquetil Typhaine, Guihot Anne-Laure, Boudou Frederic, Raymond-Letron Isabelle, Chambon Pierre, Gourdy Pierre, Ohlsson Claes, Laurell Henrik, Fontaine Coralie, Metivier Raphaël, Le Romancer Muriel, Henrion Daniel, Arnal Jean-Francois, Lenfant Francoise, ;2020;Arteriosclerosis, thrombosis, and vascular biology;40;2143-2158; 32640903
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Estrogen resistance syndrome / Orphanet_785

MGI phenotypes (gene matching)

increased white adipose tissue amount / MGI
increased bone mineral density / MGI
decreased bone mineral density / MGI
decreased compact bone thickness / MGI
abnormal vertebrae morphology / MGI
abnormal bone marrow cell number / MGI
impaired fertilization / MGI
cortical renal glomerulopathies / MGI
abnormal mammary gland development / MGI
abnormal branching of the mammary ductal tree / MGI
decreased thymocyte number / MGI
abnormal hypothalamus morphology / MGI
abnormal uterus morphology / MGI
uterus hypoplasia / MGI
abnormal ovary morphology / MGI
impaired ovarian folliculogenesis / MGI
abnormal ovarian folliculogenesis / MGI
abnormal ovarian follicle morphology / MGI
absent mature ovarian follicles / MGI
absent corpus luteum / MGI
abnormal vagina morphology / MGI
abnormal vagina epithelium morphology / MGI
abnormal testis morphology / MGI
seminiferous tubule degeneration / MGI
abnormal spermatogenesis / MGI
decreased body length / MGI
increased body weight / MGI
obese / MGI
decreased body weight / MGI
increased body size / MGI
increased aggression towards mice / MGI
increased aggression towards females / MGI
abnormal social investigation / MGI
abnormal mating receptivity / MGI
reduced male mating frequency / MGI
abnormal pup retrieval / MGI
pup cannibalization / MGI
abnormal maternal nurturing / MGI
hyperactivity / MGI
abnormal skeleton physiology / MGI
abnormal osteoclast physiology / MGI
increased circulating HDL cholesterol level / MGI
abnormal vasodilation / MGI
failure of embryo implantation / MGI
increased circulating follicle stimulating hormone level / MGI
increased circulating luteinizing hormone level / MGI
thymus hypoplasia / MGI
reduced male fertility / MGI
infertility / MGI
male infertility / MGI
female infertility / MGI
ovary cysts / MGI
abnormal skeleton development / MGI
abnormal definitive hematopoiesis / MGI
abnormal kidney physiology / MGI
abnormal B cell differentiation / MGI
abnormal reproductive system morphology / MGI
no abnormal phenotype detected / MGI
abnormal innervation / MGI
abnormal seminiferous tubule morphology / MGI
abnormal B cell number / MGI
abnormal immunoglobulin level / MGI
increased vertical activity / MGI
abnormal epididymis morphology / MGI
small uterus / MGI
asthenozoospermia / MGI
oligozoospermia / MGI
abnormal nociception after inflammation / MGI
glomerulonephritis / MGI
decreased vertical activity / MGI
short tibia / MGI
decreased circulating testosterone level / MGI
increased circulating testosterone level / MGI
decreased heart weight / MGI
increased growth rate / MGI
albuminuria / MGI
abnormal bone mineralization / MGI
increased urine protein level / MGI
no phenotypic analysis / MGI
abnormal long bone epiphyseal plate morphology / MGI
abnormal cell migration / MGI
short femur / MGI
testicular atrophy / MGI
decreased ovulation rate / MGI
impaired granulosa cell differentiation / MGI
abnormal uterus development / MGI
abnormal response/metabolism to endogenous compounds / MGI
abnormal male reproductive system physiology / MGI
abnormal female reproductive system physiology / MGI
abnormal thymus physiology / MGI
abnormal bone structure / MGI
decreased aggression towards males / MGI
decreased aggression towards mice / MGI
impaired passive avoidance behavior / MGI
abnormal vascular endothelial cell physiology / MGI
decreased bone mass / MGI
short ulna / MGI
altered response of heart to induced stress / MGI
decreased length of long bones / MGI
decreased circulating insulin-like growth factor I level / MGI
abnormal efferent ductules of testis morphology / MGI
increased anti-double stranded DNA antibody level / MGI
abnormal class switch recombination / MGI
ovary hemorrhage / MGI
abnormal testis weight / MGI
decreased testis weight / MGI
abnormal vascular wound healing / MGI
decreased energy expenditure / MGI
abnormal endometrium morphology / MGI
decreased male germ cell number / MGI
increased uterus weight / MGI
decreased uterus weight / MGI
enlarged uterus / MGI
abnormal epididymis epithelium morphology / MGI
decreased thymus weight / MGI
increased osteoclast cell number / MGI
decreased osteoblast cell number / MGI
decreased bone strength / MGI
decreased B cell number / MGI
increased double-positive T cell number / MGI
decreased circulating prolactin level / MGI
increased luteinizing hormone level / MGI
increased circulating cholesterol level / MGI
decreased circulating estradiol level / MGI
increased circulating estradiol level / MGI
decreased circulating progesterone level / MGI
impaired glucose tolerance / MGI
insulin resistance / MGI
adipose tissue phenotype / MGI
growth/size/body region phenotype / MGI
cardiovascular system phenotype / MGI
behavior/neurological phenotype / MGI
reproductive system phenotype / MGI
skeleton phenotype / MGI
hematopoietic system phenotype / MGI
abnormal circulating protein level / MGI
hyporesponsive to tactile stimuli / MGI
increased circulating glucose level / MGI
teratozoospermia / MGI
decreased lung weight / MGI
abnormal pituitary gland physiology / MGI
decreased aggression / MGI
increased circulating leptin level / MGI
decreased long bone epiphyseal plate size / MGI
abnormal rete testis morphology / MGI
dilated rete testis / MGI
decreased CD4-positive, alpha beta T cell number / MGI
decreased CD8-positive, alpha-beta T cell number / MGI
increased plasma cell number / MGI
decreased mature B cell number / MGI
decreased immature B cell number / MGI
decreased lactotroph cell number / MGI
increased spleen germinal center number / MGI
abnormal mesonephros morphology / MGI
increased IgG3 level / MGI
abnormal spleen physiology / MGI
abnormal lymph node cell ratio / MGI
anovulation / MGI
abnormal physiological response to xenobiotic / MGI
decreased physiological sensitivity to xenobiotic / MGI
detached sperm flagellum / MGI
vagina hypoplasia / MGI
absent estrous cycle / MGI
uterus cysts / MGI
endometrium hyperplasia / MGI
decreased endometrial gland number / MGI
increased white fat cell size / MGI
increased white fat cell number / MGI
dilated efferent ductules of testis / MGI
abnormal internal male genitalia morphology / MGI
enlarged endometrial glands / MGI
dilated seminiferous tubules / MGI
abnormal bone marrow cell physiology / MGI
increased gonadal fat pad weight / MGI
increased epididymal fat pad weight / MGI
increased inguinal fat pad weight / MGI
increased mammary fat pad weight / MGI
increased parametrial fat pad weight / MGI
increased renal fat pad weight / MGI
increased retroperitoneal fat pad weight / MGI
abnormal secondary ovarian follicle morphology / MGI
increased theca cell number / MGI
ovarian follicular cyst / MGI
abnormal superovulation / MGI
abnormal uterus physiology / MGI
decreased birth weight / MGI
abnormal chondrocyte physiology / MGI
spermatic granuloma / MGI
failure of ejaculation / MGI
increased total body fat amount / MGI
decreased bone mineral content / MGI
increased compact bone area / MGI
decreased compact bone area / MGI
decreased primordial ovarian follicle number / MGI
renal glomerulus hypertrophy / MGI
expanded mesangial matrix / MGI
abnormal seminiferous tubule epithelium morphology / MGI
abnormal cremaster muscle morphology / MGI
abnormal volumetric bone mineral density / MGI
increased volumetric bone mineral density / MGI
decreased volumetric bone mineral density / MGI
decreased areal bone mineral density / MGI
decreased bone ossification / MGI
decreased bone mineralization / MGI
renal glomerular immunoglobulin deposits / MGI

Literature references

Mutation of Arginine 264 on ERα (Estrogen Receptor Alpha) Selectively Abrogates the Rapid Signaling of Estradiol in the Endothelium Without Altering Fertility.;Adlanmerini Marine, Fébrissy Chanaelle, Zahreddine Rana, Vessières Emilie, Buscato Mélissa, Solinhac Romain, Favre Julie, Anquetil Typhaine, Guihot Anne-Laure, Boudou Frederic, Raymond-Letron Isabelle, Chambon Pierre, Gourdy Pierre, Ohlsson Claes, Laurell Henrik, Fontaine Coralie, Metivier Raphaël, Le Romancer Muriel, Henrion Daniel, Arnal Jean-Francois, Lenfant Francoise, ;2020;Arteriosclerosis, thrombosis, and vascular biology;40;2143-2158; 32640903

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6N(B6J)-Esr1tm3.1Ics/Ics