B6(Cg)-Abcc1tm1.1(ABCC1)Pahnk/Kctt
| Status | Available to order |
| EMMA ID | EM:16165 |
| Citation information | RRID:IMSR_EM:16165 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6(Cg)-Abcc1tm1.1(ABCC1)Pahnk/Kctt |
| Alternative name | B6(Cg)-Abcc1(tm1.1(ABCC1)Pahnk)/Pahnk |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Abcc1tm1.1(ABCC1)Pahnk |
| Gene/Transgene symbol | Abcc1 |
Information from provider
| Provider | Jens Pahnke |
| Provider affiliation | Department of Pathology, Pahnke Lab (www.pahnkelab.eu) |
| Genetic information | Abcc1 humanized floxed knock-in model. The murine exon 2 of the Abcc1 gene locus has been replaced in‑frame with the human ABCC1 coding sequence, yielding a chimeric protein that is >99.9 % human (only a single amino‑acid difference remains). The native mouse promoter and first exon are preserved to maintain physiological regulation. The human cassette is flanked by loxP sites, allowing cre recombinase‑mediated deletion of the coding sequence (hABCC1-/-). Two point mutations in exon 3 introduce stop codons in all three reading frames to prevent translation of any residual transcript after recombination. A neomycin‑resistance cassette flanked by flippase‑recognition target (FRT) sequences was introduced into the targeting vector to enable positive selection of clones, and flp‑mediated excision was achieved by crossing the founders with C57BL/6J flp‑deleter mice. |
| Phenotypic information | Homozygous:Mice homozygous for the humanized hABCC1 allele (hABCC1-flx/flx) appear phenotypically normal with respect to appearance, growth, fertility, coat condition, and overall health, and are indistinguishable from wild-type C57BL/6J controls. At the molecular level, they express human ABCC1 mRNA (lower than endogenous mouse Abcc1 in brain but higher in lung) and ABCC1 protein at levels comparable to those of wild-type mice, with approximately 60% higher expression in the brain. Functional PET imaging with 6-bromo-7-[¹¹C]methylpurine shows tracer clearance from brain and lung with kinetics similar to wild-type animals, with approximately 43% faster elimination from the brain, indicating that the human transporter is functional and replaces the murine protein. Transport remains sensitive to MK571, which increases tracer retention in both humanized and wild-type mice.Heterozygous:Heterozygous (hABCC1-flx/+) mice have not been formally evaluated, but no phenotypic or functional changes are expected. |
| Breeding history | Recipient blastocysts were harvested from B6(Cg)-Tyrc-2J/J mice. After microinjection, four C57BL/6N ES cell clones were selected for reimplantation into pseudopregnant OF1 females; 6 male chimeras derived from 3 distinct clones were generated, exhibiting |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | heterozygous C57BL/6J females |
Literature references
- Generation and Characterization of an Abcc1 Humanized Mouse Model (hABCC1flx/flx ) with Knockout Capability.;Krohn Markus, Zoufal Viktoria, Mairinger Severin, Wanek Thomas, Paarmann Kristin, Brüning Thomas, Eiriz Ivan, Brackhan Mirjam, Langer Oliver, Pahnke Jens, ;2019;Molecular pharmacology;96;138-147; 31189668