IMPC phenotypes (gene matching)
B6(Cg)-Abca7tm1.1(ABCA7)Pahnk/Kctt
| Status | Available to order |
| EMMA ID | EM:16166 |
| Citation information | RRID:IMSR_EM:16166 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6(Cg)-Abca7tm1.1(ABCA7)Pahnk/Kctt |
| Alternative name | B6J.Cg-Abca7(tm1.1(ABCA7)Pahnk)/Pahnk |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Abca7tm1.1(ABCA7)Pahnk |
| Gene/Transgene symbol | Abca7 |
Information from provider
| Provider | Jens Pahnke |
| Provider affiliation | Department of Pathology, Pahnke Lab (www.pahnkelab.eu) |
| Genetic information | Abca7 humanized floxed knock-in model. The murine Abca7 locus was humanized by in frame insertion of human type I ABCA7 cDNA (starting from human exon 2) into the 5' end of mouse Abca7 exon 4, generating a mouse/human chimeric ABCA7 protein whose N terminal portion is encoded by mouse Abca7 exons 2 and 3 and whose remaining sequence is encoded by the inserted human cDNA. An exogenous polyadenylation signal (human growth hormone polyA) was placed downstream of the human ABCA7 stop codon, and the strategy was designed to avoid deregulation of the neighboring Cnn2 gene while disrupting the endogenous mouse Abca7 locus. Two loxP sites were introduced upstream of Abca7 exon 1 and downstream of the human ABCA7 cDNA cassette, enabling cre recombinase-mediated deletion of the inserted ABCA7 cassette to generate conditional knock-out animals. The targeting vector was electroporated into C57BL/6 embryonic stem cells, correctly recombined clones were selected and validated (including Southern blot based quality control), and the allele was registered as Abca7tm1.1(ABCA7)Pahnk (MGI:6258226). A neomycin selection cassette was used during targeting, and flp-mediated excision generated a neo-excised humanized allele, as documented by Southern blot analysis and PCR-based genotyping. |
| Phenotypic information | Homozygous:Mice homozygous for the humanized hABCA7 targeted allele (hABCA7-flx/flx) are viable and can be maintained as a homozygous line on a C57BL/6 background, serving as the foundation for generating constitutive or cell type-specific Abca7 knock-outs via cre recombination. Homozygous ABCA7-flx/flx individuals appear phenotypically normal with respect to appearance, growth, fertility, coat condition, and overall health, and are indistinguishable from wild-type C57BL/6 controls. No phenotypic changes have been reported for mice carrying the floxed alleles (ABCA7-flx/flx); phenotypic effects have only been observed after cre-mediated Abca7 deletion when crossed with an amyloid-producing mouse line.Heterozygous:Heterozygous (hABCA7-flx/+) mice have not been formally evaluated, but no phenotypic or functional changes are expected. |
| Breeding history | Recipient blastocysts were harvested from C57BL/6J-Tyrc-2J/J mice. After microinjection, five C57BL/6 ES cell clones were selected for reimplantation into pseudopregnant OF1 females; 14 highly chimeric males derived from 4 distinct clones were generated, exhibiting |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | homozygous C57BL/6J females |
Disease and phenotype information
Literature references
- The ABC transporter A7 modulates neuroinflammation via NLRP3 inflammasome in Alzheimer's disease mice.;Santos-García Irene, Bascuñana Pablo, Brackhan Mirjam, Villa María, Eiriz Ivan, Brüning Thomas, Pahnke Jens, ;2025;Alzheimer's research & therapy;17;30; 39871385