B6N;129S2(Cg)-Eif2b5tm1.1Ics/Ics

Status

Available to order

EMMA IDEM:16182
Citation informationRRID:IMSR_EM:16182 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

International strain nameB6N;129S2(Cg)-Eif2b5tm1.1Ics/Ics
Alternative nameEif2b5tm1.1Ics/Ics
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolEif2b5tm1.1Ics
Gene/Transgene symbolEif2b5

Information from provider

ProviderAnne Fogli
Provider affiliationUFR de Pharmacie / UFR de Médecine et des Professions paramédicales TSA 50400
Genetic informationA loxP site was inserted into intron 2 and second loxP site followed by an FRT site-flanked neomycin resistance gene cassette were inserted into intron 7. The neo cassette was removed through subsequent flp-mediated recombination (MGI:2158498), leaving a conditional-ready allele with floxed exon 3-7 (GRCm39, Eif2b5-201).
Phenotypic informationHomozygous:
N/A

Heterozygous:
N/A
Breeding historyThe line was backcrossed 3 times with C57BL/6N mice (129S2/SvPas 12.5%, C57BL/6NTac 87.5%).
ReferencesNone available
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • decreased mean corpuscular volume / IMPC
  • increased bone mineral content / IMPC
  • abnormal bone mineralization / IMPC
  • decreased granulocyte number / IMPC
  • decreased mean corpuscular hemoglobin concentration / IMPC
  • abnormal bone structure / IMPC
  • decreased circulating glucose level / IMPC
  • increased circulating creatinine level / IMPC
  • decreased heart weight / IMPC
MGI phenotypes (gene matching)
  • abnormal forebrain morphology / MGI
  • abnormal myelination / MGI
  • demyelination / MGI
  • sporadic seizures / MGI
  • decreased body weight / MGI
  • abnormal retina morphology / MGI
  • ataxia / MGI
  • impaired coordination / MGI
  • premature death / MGI
  • abnormal astrocyte morphology / MGI
  • ectopic Bergmann glia cells / MGI
  • abnormal locomotor activation / MGI
  • abnormal microglial cell physiology / MGI
  • abnormal retinal outer nuclear layer morphology / MGI
  • abnormal retinal inner nuclear layer morphology / MGI
  • abnormal axon morphology / MGI
  • decreased percent body fat/body weight / MGI
  • brain vacuoles / MGI
  • abnormal brain white matter morphology / MGI
  • abnormal brain internal capsule morphology / MGI
  • thin retinal inner plexiform layer / MGI
  • abnormal physiological response to xenobiotic / MGI
  • abnormal astrocyte physiology / MGI
  • decreased oligodendrocyte number / MGI
  • abnormal cerebellum white matter morphology / MGI
  • decreased grip strength / MGI
  • increased oligodendrocyte number / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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