IMPC phenotypes (gene matching)
C57BL/6N-Fbn1tm2.1Ics/Ics
| Status | Available to order |
| EMMA ID | EM:16184 |
| Citation information | RRID:IMSR_EM:16184 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6N-Fbn1tm2.1Ics/Ics |
| Alternative name | Fbn1tm2.1Ics/Ics |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Fbn1tm2.1Ics |
| Gene/Transgene symbol | Fbn1 |
Information from provider
| Provider | Carine Le goff |
| Provider affiliation | Équipe n°2 : Cardiovascular structural diseases, INSERM U1148, Site hôpital Bichat |
| Genetic information | A loxP site was inserted into mouse intron 41. A lox511 site, an inverted sequence coding for the last ca. 200 bp of human intron 41 and exon 42 [ENSE00003578311.1, coding for the equivalent sequence of mouse exon 42 (ENSMUSE00000325741.2, Fbn1-201, GRCm39)] and the first ca. 200 bp of human intron 42, followed by an inverted loxP site, an FRT site-flanked neomycin resistance gene cassette and an inverted lox511 site, were inserted into mouse intron 42. The human exon contains a p.C1720Y (c.5159G>A) mutation. The neo cassette was removed through subsequent flp-mediated recombination. This allele expresses the wild-type protein and only after cre-mediated inversion of human exon 42 and deletion of mouse exon 42 it will express the mutant humanized protein. |
| Phenotypic information | Homozygous:N/AHeterozygous:N/A |
| Breeding history | C57BL/6NCrl 99.61%, C57BL/6NTac 0.39% |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France |