C57BL/6N-Rspo1tm2a(KOMP)Wtsi/Ics

Status

Available to order

EMMA IDEM:16197
Citation informationRRID:IMSR_EM:16197 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6N-Rspo1tm2a(KOMP)Wtsi/Ics
Alternative nameRspo1tm2a(KOMP)Wtsi/Ics
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolRspo1tm2a(KOMP)Wtsi
Gene/Transgene symbolRspo1

Information from provider

ProviderMarie-Christine CHABOISSIER
Provider affiliation
Genetic informationWe used the KOMP ES clone EPD0720_2_D03. The L1L2_Bact_P cassette was inserted at position 124884453 of Chrom. 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 124885446. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knock-out mouse. If cre expression occurs without flp expression, a reporter knock-out mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml.
Phenotypic informationHomozygous:
N/A

Heterozygous:
N/A
Breeding historyC57BL/6NCrl 75%, C57BL/6NTac 25%
ReferencesNone available
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

Disease and phenotype information

IMPC phenotypes (gene matching)
  • increased lean body mass / IMPC
  • increased circulating creatinine level / IMPC
  • abnormal testis morphology / IMPC
  • increased lymphocyte cell number / IMPC
  • increased grip strength / IMPC
  • decreased brain weight / IMPC
  • decreased total body fat amount / IMPC
  • small testis / IMPC
  • decreased lean body mass / IMPC
  • abnormal eye anterior chamber depth / IMPC
  • abnormal retina outer nuclear layer morphology / IMPC
  • increased bone mineral content / IMPC
  • decreased total retina thickness / IMPC
  • abnormal vitreous body morphology / IMPC
  • abnormal kidney morphology / IMPC
  • female infertility / IMPC
  • abnormal vibrissa morphology / IMPC
  • increased circulating alkaline phosphatase level / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Right strain for your research?

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