C57BL/6N-Smad4^tm1.1Ics/Ics

Status	Available to order
EMMA ID	EM:16199
Citation information	RRID:IMSR_EM:16199 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-Smad4^tm1.1Ics/Ics
Alternative name	Smad4tm1.1Ics/Ics
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Smad4^tm1.1Ics
Gene/Transgene symbol	Smad4

Information from provider

Provider	Carine Le goff
Provider affiliation	Équipe n°2 : Cardiovascular structural diseases, INSERM U1148, Site hôpital Bichat
Genetic information	A loxP site was inserted into intron 11 and a STOP cassette (containing 3x SV40 poly(A) signal sequences), an FRT site-flanked neomycin resistance gene cassette, 3' sequence of intron 11 and a modified copy of exon 12 were inserted into exon 12 (ENSMUSE00000337424, Smad4-201, GRCm39) immediately after the stop codon. The modification entails changing isoleucine codon 499 (ATT) to valine (GTT) (c.1495A>G p.I499V). The neo cassette was removed through subsequent flp-mediated recombination. This allele expresses the wild-type protein and only after cre-mediated deletion of the endogenous exon 12 coding region it will express the mutated protein.
Phenotypic information	Homozygous: N/A Heterozygous: N/A
Breeding history	C57BL/6NCrl 100%
References	None available
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Myhre syndrome / Orphanet_2588
Generalized juvenile polyposis/juvenile polyposis coli / Orphanet_329971
Familial thoracic aortic aneurysm and aortic dissection / Orphanet_91387
Hereditary hemorrhagic telangiectasia / Orphanet_774

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Right strain for your research?

The information provided on this page is, to the best of EMMA’s knowledge, based on data supplied by the original provider. End users are responsible for reviewing these details and for validating the strain and its suitability for their experimental use.
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C57BL/6N-Smad4tm1.1Ics/Ics