C57BL/6N-Chmp2btm1.1Ics/Ics

Status

Available to order

EMMA IDEM:16210
Citation informationRRID:IMSR_EM:16210 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6N-Chmp2btm1.1Ics/Ics
Alternative nameChmp2btm1.1Ics/Ics
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolChmp2btm1.1Ics
Gene/Transgene symbolChmp2b

Information from provider

ProviderFrédérique Rene
Provider affiliationCentre de Recherche en Biomédecine de Strasbourg (CRBS)
Genetic informationWe inserted into intron 4 a loxP site, the exon 5 sequence (ENSMUSE00000131914, Chmp2b-201 GRCm39), a STOP codon, an IRES sequence, the eYFP reporter sequence and a 3X pA sequence followed by a FRT-flanked neomycin resistance gene cassette and a second loxP site. The neo cassette was removed through subsequent flp-mediated recombination leading to a knock-in reporter allele of Chmp2b which can be reverted to a wild-type allele upon cre-mediated recombination.
Phenotypic informationHomozygous:
N/A

Heterozygous:
N/A
Breeding history100% C57BL/6N (C57BL/6NCrl 99,22% C57BL/6NTac 0,78%)
ReferencesNone available
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

Disease and phenotype information

IMPC phenotypes (gene matching)
  • absent seminal vesicle / IMPC
  • preweaning lethality, incomplete penetrance / IMPC
  • enlarged seminal vesicle / IMPC
  • abnormal uterus morphology / IMPC
  • no spontaneous movement / IMPC
  • enlarged spleen / IMPC
  • small testis / IMPC
  • abnormal kidney morphology / IMPC
  • decreased heart rate / IMPC
  • abnormal testis morphology / IMPC
  • abnormal liver morphology / IMPC
  • blind uterus / IMPC
  • small kidney / IMPC
  • abnormal spleen morphology / IMPC
  • prolonged RR interval / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Right strain for your research?

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