IMPC phenotypes (gene matching)
C57BL/6N-Trpa1tm1.1Ics/Ics
| Status | Available to order |
| EMMA ID | EM:16230 |
| Citation information | RRID:IMSR_EM:16230 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6N-Trpa1tm1.1Ics/Ics |
| Alternative name | Trpa1tm1.1Ics/Ics |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Trpa1tm1.1Ics |
| Gene/Transgene symbol | Trpa1 |
Information from provider
| Provider | Francesco De Lugo |
| Provider affiliation | Department of Health Sciences, Clinical Pharmacology and Oncology Unit, University of Florence |
| Genetic information | A loxP site was inserted upstream exon 22 (ENSMUSE00000457495). A lox511 site, a 531 bp synthetic DNA sequence consisting of a degenerated and inverted version of exon 22 harboring the mutation of interest flanked by human intronic sequence, a reverse loxP site, a FRT-flanked neomycin resistance cassette and a reverse lox511 site were inserted downstream exon 22. The neo cassette was removed through subsequent flp-mediated recombination leading to a conditional PM allele. Subsequent cre-mediated recombination will change asparagine codon 858 (AAC) to serine (TCT) (C.2572_2574delAACinsTCT p.N858S). |
| Phenotypic information | Homozygous:N/AHeterozygous:N/A |
| Breeding history | 100% C57BL/6NCrl |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France |