C57BL/6N-Vps13btm1.2Ics/Ics

Status

Available to order

EMMA IDEM:16232
Citation informationRRID:IMSR_EM:16232 

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International strain nameC57BL/6N-Vps13btm1.2Ics/Ics
Alternative nameVps13btm1.2Ics/Ics
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolVps13btm1.2Ics
Gene/Transgene symbolVps13b

Information from provider

ProviderChristel Thauvin-Robinet
Provider affiliationEquipe GAD, INSERM U1231, Bâtiment B3, Université de Bourgogne
Genetic informationA loxP site was inserted into intron 2 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 3. The neo cassette was removed through subsequent flp-mediated recombination. Exon 3 was deleted through subsequent cre-mediated recombination, creating a frameshift in the reading frame.
Phenotypic informationHomozygous:
N/A

Heterozygous:
N/A
Breeding history100% C57BL/6N (C57BL/6N 0,20% C57BL/6NCrl 71,88% C57BL/6NTac 27,92%)
References
  • Vps13b is required for acrosome biogenesis through functions in Golgi dynamic and membrane trafficking.;Da Costa Romain, Bordessoules Morgane, Guilleman Magali, Carmignac Virginie, Lhussiez Vincent, Courot Hortense, Bataille Amandine, Chlémaire Amandine, Bruno Céline, Fauque Patricia, Thauvin Christel, Faivre Laurence, Duplomb Laurence, ;2020;Cellular and molecular life sciences : CMLS;77;511-529; 31218450
Homozygous fertilefemales only
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

Disease and phenotype information

IMPC phenotypes (gene matching)
  • increased startle reflex / IMPC
  • abnormal lens morphology / IMPC
  • small heart / IMPC
  • hydropic degeneration / IMPC
  • abnormal eye morphology / IMPC
  • cataract / IMPC
  • vacuolation / IMPC
  • decreased exploration in new environment / IMPC
  • germ cell defect / IMPC
  • steatosis / IMPC
  • abnormal liver morphology / IMPC
  • decreased lymphocyte cell number / IMPC
  • spermatogenesis defect / IMPC
  • aspermia / IMPC
  • cataract; lens extrusion / IMPC
  • dysplasia / IMPC
  • decreased circulating alkaline phosphatase level / IMPC
  • decreased body length / IMPC
  • abnormal skin morphology / IMPC
  • male infertility / IMPC
  • decreased leukocyte cell number / IMPC
  • increased total body fat amount / IMPC
  • small spleen / IMPC
  • increased grip strength / IMPC
  • increased mean corpuscular volume / IMPC
  • enlarged lymph nodes / IMPC
  • decreased lean body mass / IMPC
  • impaired glucose tolerance / IMPC

Literature references

  • Vps13b is required for acrosome biogenesis through functions in Golgi dynamic and membrane trafficking.;Da Costa Romain, Bordessoules Morgane, Guilleman Magali, Carmignac Virginie, Lhussiez Vincent, Courot Hortense, Bataille Amandine, Chlémaire Amandine, Bruno Céline, Fauque Patricia, Thauvin Christel, Faivre Laurence, Duplomb Laurence, ;2020;Cellular and molecular life sciences : CMLS;77;511-529; 31218450

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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