IMPC phenotypes (gene matching)
B6.129S2-Hsf2tm1Mmr/Orl
| Status | Available to order |
| EMMA ID | EM:01636 |
| Citation information | RRID:IMSR_EM:01636 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129S2-Hsf2tm1Mmr/Orl |
| Alternative name | Hsf2KO |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Hsf2tm1Mmr |
| Gene/Transgene symbol | Hsf2 |
Information from provider
| Provider | Valerie Mezger |
| Provider affiliation | Ecole Normale Superieure |
| Genetic information | The Hsf2 gene was disrupted by insertion of the beta-geo gene at the 5' of exon 5 (oligomerization domain sequence), using a promoterless construct. |
| Phenotypic information | Hsf2 knock-out mice are not sterile but display meiotic defects due to the synaptonemal complex, in both genders. Males show a 60% reduction in sperm count but are fertile. Females are hypofertile and it is better to maintain the strain by breeding heterozygous individuals. Hsf2 knock-out mice also display brain abnormalities characterized by the enlargement of ventricles, reduction in hippocampus size and cortical abnormalities. |
| Breeding history | Recombinant 129 ES cells were injected into C57BL/6 blastocysts. One female transmitted the recombinant allele to its progeny. Mice were backcrossed for 8 generations to the C57BL/6 background. We cross only heterozygous individuals because of the meiotic phenotype. There is a possibility of a mix between the B6J and B6N background. |
| References |
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Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
Disease and phenotype information
MGI phenotypes (allele matching)
- abnormal ovarian follicle morphology / MGI
- absent corpus luteum / MGI
- small testis / MGI
- small seminiferous tubules / MGI
- abnormal spermatogenesis / MGI
- reduced female fertility / MGI
- decreased litter size / MGI
- decreased mature ovarian follicle number / MGI
- oligozoospermia / MGI
- maternal effect / MGI
- ovary hemorrhage / MGI
- decreased testis weight / MGI
- decreased epididymis weight / MGI
- abnormal male meiosis / MGI
- decreased oocyte number / MGI
- abnormal female germ cell morphology / MGI
- abnormal male germ cell apoptosis / MGI
- small hippocampus / MGI
- enlarged lateral ventricles / MGI
- enlarged third ventricle / MGI
- ovarian follicular cyst / MGI
- abnormal synaptonemal complex / MGI
MGI phenotypes (gene matching)
- dilated lateral ventricles / MGI
- dilated third ventricle / MGI
- abnormal ovarian follicle morphology / MGI
- absent corpus luteum / MGI
- small testis / MGI
- small seminiferous tubules / MGI
- abnormal spermatogenesis / MGI
- intracerebral hemorrhage / MGI
- reduced female fertility / MGI
- decreased litter size / MGI
- no abnormal phenotype detected / MGI
- abnormal seminiferous tubule morphology / MGI
- decreased mature ovarian follicle number / MGI
- oligozoospermia / MGI
- maternal effect / MGI
- ovary hemorrhage / MGI
- decreased testis weight / MGI
- decreased male germ cell number / MGI
- decreased epididymis weight / MGI
- abnormal male meiosis / MGI
- decreased oocyte number / MGI
- abnormal female germ cell morphology / MGI
- abnormal male germ cell apoptosis / MGI
- small hippocampus / MGI
- enlarged lateral ventricles / MGI
- enlarged third ventricle / MGI
- ovarian follicular cyst / MGI
- abnormal synaptonemal complex / MGI
- postnatal lethality, incomplete penetrance / MGI
- prenatal lethality, incomplete penetrance / MGI
- collapsed brain ventricles / MGI
Literature references
- Genomic structure and chromosomal localization of the mouse Hsf2 gene and promoter sequences.;Manuel M, Sage J, Mattéi M G, Morange M, Mezger V, ;1999;Gene;232;115-24; 10333528
- Brain abnormalities, defective meiotic chromosome synapsis and female subfertility in HSF2 null mice.;Kallio Marko, Chang Yunhua, Manuel Martine, Alastalo Tero-Pekka, Rallu Murielle, Gitton Yorick, Pirkkala Lila, Loones Marie-Thérèse, Paslaru Liliana, Larney Severine, Hiard Sophie, Morange Michel, Sistonen Lea, Mezger Valérie, ;2002;The EMBO journal;21;2591-601; 12032072
- Phenotypic characterization of mouse embryonic fibroblasts lacking heat shock factor 2.;Paslaru Liliana, Morange M, Mezger Valérie, ;2003;Journal of cellular and molecular medicine;7;425-35; 14754511