C.Cg-Rag2tm1Fwa Tg(Tcra/Tcrb)1Vbo/Ieg

Status

Available to order

EMMA IDEM:16731
Citation informationRRID:IMSR_EM:16731 

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International strain nameC.Cg-Rag2tm1Fwa Tg(Tcra/Tcrb)1Vbo/Ieg
Alternative nameC.Rag2-tm1Fwa Tg(HA-TCRa,HA-TCRb)17Bii
Strain typeTransgenic Strains
Allele/Transgene symbolTg(Tcra/Tcrb)1Vbo, Rag2tm1Fwa
Gene/Transgene symbolTg(Tcra/Tcrb)1Vbo, Rag2

Information from provider

ProviderJörg Kirberg
Provider affiliationIMG53, Paul-Ehrlich-Institut
Genetic informationMice are homozygous for the Rag2 knock-out allele and for the TCR transgene, which has specificity for the hemagglutinin (HA) peptide in the context of I-Ed class II MHC molecules. Rag2tm1Fwa: targeting in CCE ES-cells, thus Rag2 knock-out allele on 129S/SvEv-Gpi1c background (Shinkai Y, Koyasu S, Nakayama K, Murphy KM, Loh DY, Reinherz EL, Alt FW, Science. 1993 259(5096):822-5). Tg(HA-TCRa,HA-TCRb)17Bii (MGI transgene: Tg(Tcra/Tcrb)1Vbo, MGI:2680176) Transgenesis by pronuclear injection into (B6 x DBA/2)F2 fertilized eggs (Kirberg, J., A. Baron, S. Jakob, A. Rolink, K. Karjalainen, and H. von Boehmer. Thymic selection of CD8+ single positive cells with a class II major histocompatibility complex-restricted receptor. 1994, J Exp Med 180:25-34).
Phenotypic informationHomozygous:
With Rag2 knock-out homozygosity, all T cells must use the transgene-encoded HA-TCR (they are TCR monoclonal), thus no T cells with other TCRs are present. Mice will have no B cells due to Rag2 knock-out homozygosity.

Heterozygous:
With Rag2 knock-out heterozygosity, all T cells will have the transgene-encoded HA-TCR, but they may also rearrange the endogenous TCRa (sometimes also TCRb) loci and express these together with the transgenic HA-TCR. In both homo- and heterozygous mice the TCR transgene is expressed in T cells.
Breeding historyMice are on BALB/cByJ (JAX strain) genetic background (N>>10).
References
  • RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.;Shinkai Y, Rathbun G, Lam K P, Oltz E M, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall A M, ;1992;Cell;68;855-67; 1547487
  • Thymic selection of CD8+ single positive cells with a class II major histocompatibility complex-restricted receptor.;Kirberg J, Baron A, Jakob S, Rolink A, Karjalainen K, von Boehmer H, ;1994;The Journal of experimental medicine;180;25-34; 8006585
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedyes

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivinghomozygous BALB/c males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • increased granulocyte number / MGI
  • abnormal spleen morphology / MGI
  • spleen hypoplasia / MGI
  • decreased body size / MGI
  • arrested B cell differentiation / MGI
  • decreased IgM level / MGI
  • thymus hypoplasia / MGI
  • arrested T cell differentiation / MGI
  • increased susceptibility to infection / MGI
  • abnormal effector T cell morphology / MGI
  • increased natural killer cell mediated cytotoxicity / MGI
  • decreased double-positive T cell number / MGI
  • increased macrophage cell number / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • lymph node hypoplasia / MGI
  • absent mature B cells / MGI
  • abnormal T cell receptor V(D)J recombination / MGI
  • abnormal immunoglobulin V(D)J recombination / MGI
  • decreased thymocyte number / MGI
  • lung inflammation / MGI
  • increased susceptibility to bacterial infection / MGI
  • abnormal macrophage physiology / MGI
  • colitis / MGI
  • abnormal T-helper 1 physiology / MGI
  • abnormal response to transplant / MGI
  • abnormal interferon secretion / MGI
  • abnormal chemokine secretion / MGI
  • decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
  • abnormal intestinal mucosa morphology / MGI
  • abnormal B cell morphology / MGI
  • decreased susceptibility to parasitic infection / MGI
  • decreased double-negative T cell number / MGI
  • increased double-negative T cell number / MGI
  • decreased susceptibility to type IV hypersensitivity reaction / MGI
  • abnormal stomach mucosa morphology / MGI
  • abnormal intestinal epithelium morphology / MGI
  • abnormal B cell number / MGI
  • decreased pre-B cell number / MGI
  • decreased mature B cell number / MGI
  • increased immature B cell number / MGI
  • decreased immature B cell number / MGI
  • abnormal gamma-delta T cell differentiation / MGI
MGI phenotypes (gene matching)
  • decreased monocyte cell number / MGI
  • increased granulocyte number / MGI
  • decreased bone marrow cell number / MGI
  • alopecia / MGI
  • abnormal digestive system morphology / MGI
  • abnormal intestinal epithelium morphology / MGI
  • abnormal intestinal mucosa morphology / MGI
  • abnormal spleen morphology / MGI
  • small spleen / MGI
  • spleen hypoplasia / MGI
  • abnormal Peyer's patch morphology / MGI
  • abnormal thymus morphology / MGI
  • decreased thymocyte number / MGI
  • abnormal immune system cell morphology / MGI
  • decreased body size / MGI
  • abnormal humoral immune response / MGI
  • arrested B cell differentiation / MGI
  • decreased IgG level / MGI
  • decreased IgM level / MGI
  • decreased IgA level / MGI
  • thymus hypoplasia / MGI
  • arrested T cell differentiation / MGI
  • lung inflammation / MGI
  • neoplasm / MGI
  • abnormal B cell differentiation / MGI
  • no abnormal phenotype detected / MGI
  • small lymph nodes / MGI
  • abnormal lymph node morphology / MGI
  • abnormal lymphopoiesis / MGI
  • abnormal pre-B cell morphology / MGI
  • increased susceptibility to infection / MGI
  • abnormal double-negative T cell morphology / MGI
  • abnormal double-positive T cell morphology / MGI
  • increased susceptibility to bacterial infection / MGI
  • abnormal effector T cell morphology / MGI
  • abnormal macrophage physiology / MGI
  • abnormal B cell number / MGI
  • abnormal B cell physiology / MGI
  • abnormal immune system organ morphology / MGI
  • colitis / MGI
  • absent Peyer's patches / MGI
  • increased natural killer cell mediated cytotoxicity / MGI
  • erythroderma / MGI
  • abnormal lymphocyte physiology / MGI
  • abnormal B cell morphology / MGI
  • decreased lymphocyte cell number / MGI
  • decreased B cell number / MGI
  • decreased T cell number / MGI
  • decreased susceptibility to parasitic infection / MGI
  • decreased double-negative T cell number / MGI
  • increased double-negative T cell number / MGI
  • decreased double-positive T cell number / MGI
  • decreased T cell proliferation / MGI
  • cachexia / MGI
  • increased macrophage cell number / MGI
  • abnormal T-helper 1 physiology / MGI
  • decreased susceptibility to type IV hypersensitivity reaction / MGI
  • abnormal response to transplant / MGI
  • abnormal T cell morphology / MGI
  • decreased NK T cell number / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • lymph node hypoplasia / MGI
  • decreased pre-B cell number / MGI
  • decreased mature B cell number / MGI
  • absent mature B cells / MGI
  • increased immature B cell number / MGI
  • decreased immature B cell number / MGI
  • abnormal gamma-delta T cell differentiation / MGI
  • decreased spleen white pulp amount / MGI
  • abnormal interferon secretion / MGI
  • abnormal chemokine secretion / MGI
  • abnormal T cell receptor V(D)J recombination / MGI
  • abnormal immunoglobulin V(D)J recombination / MGI
  • absent Hassall's corpuscle / MGI
  • small inguinal lymph nodes / MGI
  • decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
  • increased DN3 thymocyte number / MGI
  • abnormal stomach mucosa morphology / MGI
  • small cervical lymph nodes / MGI

Literature references

  • RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.;Shinkai Y, Rathbun G, Lam K P, Oltz E M, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall A M, ;1992;Cell;68;855-67; 1547487
  • Thymic selection of CD8+ single positive cells with a class II major histocompatibility complex-restricted receptor.;Kirberg J, Baron A, Jakob S, Rolink A, Karjalainen K, von Boehmer H, ;1994;The Journal of experimental medicine;180;25-34; 8006585

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