C57BL/6N-Tg(Cnp-tdTomato*)CJhi/Ieg
| Status | Available to order |
| EMMA ID | EM:16743 |
| Citation information | RRID:IMSR_EM:16743 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6N-Tg(Cnp-tdTomato*)CJhi/Ieg |
| Alternative name | C57BL/6N-Tg(Cnp-tdTomato*)CJhi/Jhi |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Cnp-tdTomato* / Cnp-tdTomato-CAAX |
| Gene/Transgene symbol | Cnp-tdTomato* / Cnp-tdTomato-CAAX |
Information from provider
| Provider | Johannes Hirrlinger |
| Provider affiliation | Carl-Ludwig-Institute for Physiology, Leipzig University |
| Genetic information | Transgenic mouse line expressing a membrane-targeted variant of the red fluorescent protein tdTomato in myelinating glial cells (oligodendrocytes, Schwann cells) driven by the mouse Cnp-promoter. For membrane association a CAAX-membrane targeting sequence coding the human H-Ras prenylation signal (Hancock et al., 1989, doi:10.1016/0092-8674(89)90054-8) was fused to the 3’-end of the tdTomato open reading frame. The transgene used has a total size of 5734 bp and contains the following genetic elements from 5’ to 3’ (location in bp): Cnp-promoter (22-3940), Kozak sequence (3965-3970), tdTomato (3971-5398), CAAX membrane anchor (5399-5458), Stop codon (5459-5461). SV40 late pA (5506-5727). The transgene construct was injected in oocytes of C57BL/6N mice. Seven mouse lines were established from this pronuclear injection, which differ in their expression pattern. This submission refers to line C, showing a sparse expression of the red-fluorescent protein in myelinating cells and in myelin. The allele has been registered at MGI as Tg(Cnp-tdTomato*)CJhi [MGI: 8278530] The mouse line has been registered at MGI as C57BL/6N-Tg(Cnp-tdTomato*)CJhi/Jhi [MGI: 8278545] br bDisclaimer:/b Multiple sublines with different expression patterns have been archived for this mutation (EM:16742, EM:16743, EM:16744 and EM:16745). As the transgene integration site is unknown, PCR-based genotyping cannot discriminate between these sublines. Consequently, the archived subline is based on the animals provided by the depositor, and its identity cannot be independently confirmed by genotyping. Recipients are therefore advised to verify the expression pattern before use if this characteristic is critical to their intended application. |
| Phenotypic information | Homozygous:Mice were not bred intentionally for homozygosity, in part due to the lack of PCR tools to discriminated between homozygous and heterozygous transgenic animals. However, no difference in the phenotype between mice which could be potentially homozygous (tg x tg breeding) and heterozygous mice was observed. Please refer to the description of heterozygous mice for further information.Heterozygous:Transgenic mice express the membrane-targeted variant of tdTomato in oligodendrocytes and Schwann cells, thereby labelling myelin with bright red fluorescence. Besides the fluorescent labelling of myelin, no further phenotype different from wild type mice is observed. In this line (C), sparse expression of tdTomato in some oligodendrocytes is observed, allowing to visualize individual oligodendrocytes and the myelin sheaths associated to a single cell. |
| Breeding history | Mice were first bred to C57BL/6N wildtype mice, followed by breeding to littermates after establishing of the colony. |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |