C.129P2-Krt10^tm2Tmm/Ieg

Status	Available to order
EMMA ID	EM:01741
Citation information	RRID:IMSR_EM:01741 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C.129P2-Krt10^tm2Tmm/Ieg
Alternative name	K10(-/-) homozygous
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Krt10^tm2Tmm
Gene/Transgene symbol	Krt10

Information from provider

Provider	Thomas Magin
Provider affiliation	Institute of physiological chemistry / Dept. of cellular biochemistry, University of Bonn
Genetic information	A PGK-HPRT minigene replaced 414 bp of the promoter region and exons 1 and 2 of the keratin 10 (Krt10) gene via homologous recombination in ES cells.
Phenotypic information	Krt10 -/- mice were born at the expected Mendelian ratio, were fully viable, and showed no obvious skin defect resulting from birth stress. Histological analysis of neonatal skin revealed normal stratification and an unaltered number of epidermal cell layers, although a slight increase in granular cell size and the size of keratohyalin granules and a loose appearance of the stratum corneum was observed. No cytolysis in Krt10 -/- epidermis was seen, suggesting that filaments consisting of Krt10 and 1 are not essential for maintaining the integrity of suprabasal epidermis in these mice. Thus, most probably a compensation by basal keratins 5, 14, and 15 prevents cytolysis and permits normal epidermal differentiation in Krt10 -/- mice.
Breeding history	Offspring of the chimaeras were mated to BALB/c mice for further analysis. K10-/- mice have been backcrossed to the BALB/c background for 6 generations.
References	Formation of a normal epidermis supported by increased stability of keratins 5 and 14 in keratin 10 null mice.;Reichelt J, Büssow H, Grund C, Magin T M, ;2001;Molecular biology of the cell;12;1557-68; 11408568 Hyperproliferation, induction of c-Myc and 14-3-3sigma, but no cell fragility in keratin-10-null mice.;Reichelt Julia, Magin Thomas M, ;2002;Journal of cell science;115;2639-50; 12077355 Loss of keratin 10 leads to mitogen-activated protein kinase (MAPK) activation, increased keratinocyte turnover, and decreased tumor formation in mice.;Reichelt Julia, Furstenberger Gerhard, Magin Thomas M, ;2004;The Journal of investigative dermatology;123;973-81; 15482487

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Autosomal dominant epidermolytic ichthyosis / Orphanet_312
Autosomal recessive epidermolytic ichthyosis / Orphanet_512103
Annular epidermolytic ichthyosis / Orphanet_281139
Congenital reticular ichthyosiform erythroderma / Orphanet_281190

MGI phenotypes (allele matching)

ruffled hair / MGI
enlarged sebaceous gland / MGI
abnormal epidermal layer morphology / MGI
abnormal epidermis suprabasal layer morphology / MGI
abnormal epidermis stratum granulosum morphology / MGI
abnormal epidermis stratum corneum morphology / MGI
decreased incidence of induced tumors / MGI
abnormal sebaceous lipid secretion / MGI
greasy coat / MGI
abnormal stratum corneum lipid matrix formation / MGI
increased keratohyalin granule size / MGI
increased sebum secretion / MGI
abnormal sebocyte morphology / MGI
abnormal sebocyte differentiation / MGI
increased sebocyte proliferation / MGI
epidermal hyperplasia / MGI
hypergranulosis / MGI

MGI phenotypes (gene matching)

scaly ears / MGI
ruffled hair / MGI
abnormal autopod morphology / MGI
abnormal foot pad morphology / MGI
enlarged sebaceous gland / MGI
reddish skin / MGI
scaly skin / MGI
flaky skin / MGI
translucent skin / MGI
blistering / MGI
abnormal epidermal layer morphology / MGI
epidermal hyperplasia / MGI
abnormal epidermis stratum basale morphology / MGI
abnormal epidermis suprabasal layer morphology / MGI
abnormal epidermis stratum spinosum morphology / MGI
abnormal epidermis stratum granulosum morphology / MGI
abnormal epidermis stratum corneum morphology / MGI
hyperkeratosis / MGI
decreased body weight / MGI
acanthosis / MGI
decreased incidence of induced tumors / MGI
abnormal skin morphology / MGI
abnormal tail morphology / MGI
increased susceptibility to infection / MGI
abnormal sebaceous lipid secretion / MGI
decreased skin tensile strength / MGI
greasy coat / MGI
abnormal stratum corneum lipid matrix formation / MGI
hypergranulosis / MGI
increased keratohyalin granule size / MGI
neonatal lethality, complete penetrance / MGI
increased sebum secretion / MGI
abnormal sebocyte morphology / MGI
abnormal sebocyte differentiation / MGI
increased sebocyte proliferation / MGI

Literature references

Formation of a normal epidermis supported by increased stability of keratins 5 and 14 in keratin 10 null mice.;Reichelt J, Büssow H, Grund C, Magin T M, ;2001;Molecular biology of the cell;12;1557-68; 11408568
Hyperproliferation, induction of c-Myc and 14-3-3sigma, but no cell fragility in keratin-10-null mice.;Reichelt Julia, Magin Thomas M, ;2002;Journal of cell science;115;2639-50; 12077355
Loss of keratin 10 leads to mitogen-activated protein kinase (MAPK) activation, increased keratinocyte turnover, and decreased tumor formation in mice.;Reichelt Julia, Furstenberger Gerhard, Magin Thomas M, ;2004;The Journal of investigative dermatology;123;973-81; 15482487

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C.129P2-Krt10tm2Tmm/Ieg