MGI phenotypes (allele matching)
- enlarged liver / MGI
C.129P2-Krt18tm1Tmm/Ieg
| Status | Available to order |
| EMMA ID | EM:01743 |
| Citation information | RRID:IMSR_EM:01743 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C.129P2-Krt18tm1Tmm/Ieg |
| Alternative name | K18(-/-) knock-out |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Krt18tm1Tmm |
| Gene/Transgene symbol | Krt18 |
Information from provider
| Provider | Thomas Magin |
| Provider affiliation | Institute of physiological chemistry / Dept. of cellular biochemistry, University of Bonn |
| Genetic information | An Hprt minigene replaced most of exon 1, intron 1 and exon 2 of the keratin 18 (Krt18) gene via homologous recombination in ES cells. |
| Phenotypic information | Krt18 null mice are viable, fertile, and show a normal lifespan. In young Krt18 null mice, hepatocytes were completely devoid of keratin filaments. Old Krt18 null mice, however, developed a distinctive liver pathology with abnormal hepatocytes containing Krt8-positive aggregates. These stained positively for ubiquitin and MM120-1 Mallory antigen and were identified as Mallory bodies, one hallmark of human alcoholic hepatitis. Another striking finding was the absence or drastic down-regulation of Krt7 in several tissues despite its ongoing transcription. However, double-deficiency for Krt18 and Krt19 led to complete loss of keratin filaments in early mouse development. These embryos died at around day E9.5 with 100% penetrance (Hesse et al., 2000). |
| Breeding history | Offspring of the chimaeras were mated to BALB/c mice for further analysis. K18-/- mice have been backcrossed to the BALB/c background for 7 generations. |
| References |
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Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
Disease and phenotype information
MGI phenotypes (gene matching)
- enlarged liver / MGI
Literature references
- Lessons from keratin 18 knockout mice: formation of novel keratin filaments, secondary loss of keratin 7 and accumulation of liver-specific keratin 8-positive aggregates.;Magin T M, Schröder R, Leitgeb S, Wanninger F, Zatloukal K, Grund C, Melton D W, ;1998;The Journal of cell biology;140;1441-51; 9508776
- Targeted deletion of keratins 18 and 19 leads to trophoblast fragility and early embryonic lethality.;Hesse M, Franz T, Tamai Y, Taketo M M, Magin T M, ;2000;The EMBO journal;19;5060-70; 11013209