B6.129S2-Lat2^tm1Mal/Orl

Status	Available to order
EMMA ID	EM:01813
Citation information	RRID:IMSR_EM:01813 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.129S2-Lat2^tm1Mal/Orl
Alternative name	NTAL KO
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Lat2^tm1Mal
Gene/Transgene symbol	Lat2

Information from provider

Provider	Malissen Marie
Provider affiliation	Centre d'Immunologi de Marseille Luminy
Genetic information	Mutation and construct described in Volná P. et al., 2004 (PubMed ID: 15477348). Insertion of a loxP-neomycin cassette with deletion of exons 2-9. Lat2 knock-out mutants produce no protein.
Phenotypic information	The NTAL/LAB (Lat2) adaptor molecule is highly expressed in mast cells and B cells. In mast cells NTAL/LAB has both a positive and a negative regulatory function in FcEpsilonRI (high-affinity IgE receptor)-mediated signaling. NTAL/LAB also plays a regulatory role in receptor-specific B cells activation.
Breeding history	This strain was first crossed onto a cre recombinase-deleter strain, followed by 6 backcrosses to C57BL/6.
References	Multiplicity and plasticity of natural killer cell signaling pathways.;Chiesa Sabrina, Mingueneau Michael, Fuseri Nicolas, Malissen Bernard, Raulet David H, Malissen Marie, Vivier Eric, Tomasello Elena, ;2006;Blood;107;2364-72; 16291591 Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL.;Volná Petra, Lebduska Pavel, Dráberová Lubica, Símová Sárka, Heneberg Petr, Boubelík Michael, Bugajev Viktor, Malissen Bernard, Wilson Bridget S, Horejsí Václav, Malissen Marie, Dráber Petr, ;2004;The Journal of experimental medicine;200;1001-13; 15477348 Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling.;Brdicka Tomás, Imrich Martin, Angelisová Pavla, Brdicková Nadezda, Horváth Ondrej, Spicka Jirí, Hilgert Ivan, Lusková Petra, Dráber Petr, Novák Petr, Engels Niklas, Wienands Jürgen, Simeoni Luca, Osterreicher Jan, Aguado Enrique, Malissen Marie, Schraven Burkhart, Horejsí Václav, ;2002;The Journal of experimental medicine;196;1617-26; 12486104 Positive and negative regulation of FcepsilonRI-mediated signaling by the adaptor protein LAB/NTAL.;Zhu Minghua, Liu Yan, Koonpaew Surapong, Granillo Olivia, Zhang Weiguo, ;2004;The Journal of experimental medicine;200;991-1000; 15477350 Single and combined deletions of the NTAL/LAB and LAT adaptors minimally affect B-cell development and function.;Wang Ying, Horvath Ondrej, Hamm-Baarke Andrea, Richelme Mireille, Grégoire Claude, Guinamard Rodolphe, Horejsi Vaclav, Angelisova Pavla, Spicka Jiri, Schraven Burkhart, Malissen Bernard, Malissen Marie, ;2005;Molecular and cellular biology;25;4455-65; 15899851 LAB: a new membrane-associated adaptor molecule in B cell activation.;Janssen Erin, Zhu Minghua, Zhang Weijia, Koonpaew Surapong, Zhang Weiguo, ;2003;Nature immunology;4;117-23; 12514734

Information from EMMA

Archiving centre	CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France

Disease and phenotype information

MGI phenotypes (allele matching)

increased anti-nuclear antigen antibody level / MGI
abnormal mast cell physiology / MGI

MGI phenotypes (gene matching)

enlarged spleen / MGI
spleen hyperplasia / MGI
abnormal mast cell physiology / MGI
abnormal T cell physiology / MGI
abnormal cytokine secretion / MGI
increased autoantibody level / MGI
abnormal CD8-positive, alpha-beta T cell physiology / MGI
increased anti-double stranded DNA antibody level / MGI
increased anti-nuclear antigen antibody level / MGI
increased T cell proliferation / MGI
immune system phenotype / MGI
increased memory T cell number / MGI
increased circulating tumor necrosis factor level / MGI
increased interferon-gamma secretion / MGI
increased circulating interleukin-6 level / MGI
increased interleukin-10 secretion / MGI
decreased interleukin-12 secretion / MGI
increased susceptibility to endotoxin shock / MGI
increased mast cell degranulation / MGI
increased sensitivity to xenobiotic induced morbidity/mortality / MGI

Literature references

Multiplicity and plasticity of natural killer cell signaling pathways.;Chiesa Sabrina, Mingueneau Michael, Fuseri Nicolas, Malissen Bernard, Raulet David H, Malissen Marie, Vivier Eric, Tomasello Elena, ;2006;Blood;107;2364-72; 16291591
Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL.;Volná Petra, Lebduska Pavel, Dráberová Lubica, Símová Sárka, Heneberg Petr, Boubelík Michael, Bugajev Viktor, Malissen Bernard, Wilson Bridget S, Horejsí Václav, Malissen Marie, Dráber Petr, ;2004;The Journal of experimental medicine;200;1001-13; 15477348
Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling.;Brdicka Tomás, Imrich Martin, Angelisová Pavla, Brdicková Nadezda, Horváth Ondrej, Spicka Jirí, Hilgert Ivan, Lusková Petra, Dráber Petr, Novák Petr, Engels Niklas, Wienands Jürgen, Simeoni Luca, Osterreicher Jan, Aguado Enrique, Malissen Marie, Schraven Burkhart, Horejsí Václav, ;2002;The Journal of experimental medicine;196;1617-26; 12486104
Positive and negative regulation of FcepsilonRI-mediated signaling by the adaptor protein LAB/NTAL.;Zhu Minghua, Liu Yan, Koonpaew Surapong, Granillo Olivia, Zhang Weiguo, ;2004;The Journal of experimental medicine;200;991-1000; 15477350
Single and combined deletions of the NTAL/LAB and LAT adaptors minimally affect B-cell development and function.;Wang Ying, Horvath Ondrej, Hamm-Baarke Andrea, Richelme Mireille, Grégoire Claude, Guinamard Rodolphe, Horejsi Vaclav, Angelisova Pavla, Spicka Jiri, Schraven Burkhart, Malissen Bernard, Malissen Marie, ;2005;Molecular and cellular biology;25;4455-65; 15899851
LAB: a new membrane-associated adaptor molecule in B cell activation.;Janssen Erin, Zhu Minghua, Zhang Weijia, Koonpaew Surapong, Zhang Weiguo, ;2003;Nature immunology;4;117-23; 12514734

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B6.129S2-Lat2tm1Mal/Orl