| Status | Available to order |
| EMMA ID | EM:01817 |
| Citation information | RRID:IMSR_EM:01817 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | 129S2/SvPas-Vezttm1Smc/Orl |
| Alternative name | vezatinFloxed/Floxed |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Vezttm1Smc |
| Gene/Transgene symbol | Vezt |
| Provider | SIMMLER MARIE-CHRISTINE |
| Provider affiliation | Institut Jacques-Monod, CNRS, Equipe labellisée INSERM U950 |
| Genetic information | The mouse vezatin gene encodes several cDNA isoforms which all start at the same ATG and all contain exon 5. Vezatin is an integral protein associated to E-cadherin adherens junctions (Küssel-Anderman et al., 2000; Sousa et al., 2004; Hyenne et al., 2005; Balhoul, Simmler et al., 2009). Exon 5 encodes the transmembrane domain. We generated a vezatin allele lacking exon 5 by using a cre/loxP strategy. The vezatin targeting vector contains a 4.5 Kb EcoRI genomic fragment, a 0.7 Kb genotyping fragment (containing exon 5) flanked by a loxP site, and a 2.4 Kb ClaI/KpnI genomic fragment. A floxed pPgk-1-neo-pA cassette inserted on the right side of exon 5-fragment and a pPgk-1-DTA-pA cassette inserted on the edge of the construct were used for positive and negative selection respectively (Hyenne V et al., Mech Dev, 124, 449-462, 2007). |
| Phenotypic information | Various cre recombinase-expressing lines used for targeting the deletion in epithelial tissues that are relevant to the description of the role of vezatin in morphogenesis. A phenotype of early embryonic lethality (E4.5-E5.5) was obtained by using the Pgk1-cre line (Lallemand Y et al. 1998) and the conditional vezatin floxed line (mixed background), described in Hyenne V et al., 2007. Homozygous vezatin flox/flox mice are fully normal and fertile. |
| References |
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| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
