B6.Cg-Tg(Lck-CFLAR)1Zrng/Ieg

Status	Available to order
EMMA ID	EM:01919
Citation information	RRID:IMSR_EM:01919 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.Cg-Tg(Lck-CFLAR)1Zrng/Ieg
Alternative name	lck FLIP short
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Lck-CFLAR)1Zrng
Gene/Transgene symbol	Tg(Lck-CFLAR)1Zrng

Information from provider

Provider	Martin Zoernig
Provider affiliation	Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus, University of Frankfurt
Genetic information	The FLAG-tagged human c-FLIP short cDNA (encoding the short form of human CASP8 and FADD-like apoptosis regulator, CFLAR or FLIP) was cloned blunt-ended into the BamHI site of the p1017 vector under control of the proximal Lck promoter for T cell-specific expression.
Phenotypic information	The heterozygous transgenic mice appear normal. Thymic and splenic cell numbers as well as CD4/CD8 cellularity are normal in Lck-FLIP short transgenic animals; c-FLIP short overexpression leads to a significant decrease in activation-induced T-cell proliferation in vitro.
Breeding history	The Lck FLIP short transgenic mouse strain is constantly bred onto the C57BL/6 background since more than 10 generations.
References	Transgenic overexpression of the Caspase-8 inhibitor FLIP(short) leads to impaired T cell proliferation and an increased memory T cell pool after staphylococcal enterotoxin B injection.;Oehme Ina, Neumann Frank, Bösser Susanne, Zörnig Martin, ;2005;European journal of immunology;35;1240-9; 15761846

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Literature references

Transgenic overexpression of the Caspase-8 inhibitor FLIP(short) leads to impaired T cell proliferation and an increased memory T cell pool after staphylococcal enterotoxin B injection.;Oehme Ina, Neumann Frank, Bösser Susanne, Zörnig Martin, ;2005;European journal of immunology;35;1240-9; 15761846

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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