IMPC phenotypes (gene matching)
C.129P2(B6)-Bclaf1tm1Lfmp/Ieg
| Status | Available to order |
| EMMA ID | EM:01979 |
| Citation information | RRID:IMSR_EM:01979 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C.129P2(B6)-Bclaf1tm1Lfmp/Ieg |
| Alternative name | Bclaf1 RK BalbC |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Bclaf1tm1Lfmp |
| Gene/Transgene symbol | Bclaf1 |
Information from provider
| Provider | Rosel Blasig |
| Provider affiliation | Research Institute of Molecular Pharmacology |
| Genetic information | Promoterless reporter gene (NotI-NheI fragment of the vector pSAßGeolox2PGKDTA) containing a loxP-flanked cassette of splice acceptor, beta-galactosidase/lacZ gene and neo gene, inserted into Bclaf1 intron between exon 3 (translation start at ATG) and exon 4 by targeted mutagenesis. Phenotypic defects due to random integration were excluded. |
| Phenotypic information | Homozygous mice normally die at the age of 2-4 weeks, some die postnatally or as embryo. Mice are pathologically normal despite half of normal body weight and retarded development of some organs or cells (brain, testis, kidney, blood cells, reduced hematopoiesis in bone marrow). |
| Breeding history | 3 backgrounds are available: mixed background 129 x C57BL/6 or 10 generations backcrossed to C57BL/6 and 10 generations backcrossed to BALB/c, inbred. |
| References |
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Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
Disease and phenotype information
MGI phenotypes (allele matching)
- no phenotypic analysis / MGI
MGI phenotypes (gene matching)
- polydactyly / MGI
- abnormal lung development / MGI
- decreased body size / MGI
- no phenotypic analysis / MGI
- increased B cell number / MGI
- decreased T cell proliferation / MGI
- decreased CD8-positive, alpha-beta T cell number / MGI
- absent gastric milk in neonates / MGI
- postnatal lethality, complete penetrance / MGI
- thick lung-associated mesenchyme / MGI
- abnormal mesenchymal cell differentiation involved in lung development / MGI
Literature references
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.;Strausberg Robert L, Feingold Elise A, Grouse Lynette H, Derge Jeffery G, Klausner Richard D, Collins Francis S, Wagner Lukas, Shenmen Carolyn M, Schuler Gregory D, Altschul Stephen F, Zeeberg Barry, Buetow Kenneth H, Schaefer Carl F, Bhat Narayan K, Hopkins Ralph F, Jordan Heather, Moore Troy, Max Steve I, Wang Jun, Hsieh Florence, Diatchenko Luda, Marusina Kate, Farmer Andrew A, Rubin Gerald M, Hong Ling, Stapleton Mark, Soares M Bento, Bonaldo Maria F, Casavant Tom L, Scheetz Todd E, Brownstein Michael J, Usdin Ted B, Toshiyuki Shiraki, Carninci Piero, Prange Christa, Raha Sam S, Loquellano Naomi A, Peters Garrick J, Abramson Rick D, Mullahy Sara J, Bosak Stephanie A, McEwan Paul J, McKernan Kevin J, Malek Joel A, Gunaratne Preethi H, Richards Stephen, Worley Kim C, Hale Sarah, Garcia Angela M, Gay Laura J, Hulyk Stephen W, Villalon Debbie K, Muzny Donna M, Sodergren Erica J, Lu Xiuhua, Gibbs Richard A, Fahey Jessica, Helton Erin, Ketteman Mark, Madan Anuradha, Rodrigues Stephanie, Sanchez Amy, Whiting Michelle, Madan Anup, Young Alice C, Shevchenko Yuriy, Bouffard Gerard G, Blakesley Robert W, Touchman Jeffrey W, Green Eric D, Dickson Mark C, Rodriguez Alex C, Grimwood Jane, Schmutz Jeremy, Myers Richard M, Butterfield Yaron S N, Krzywinski Martin I, Skalska Ursula, Smailus Duane E, Schnerch Angelique, Schein Jacqueline E, Jones Steven J M, Marra Marco A, null null, ;2002;Proceedings of the National Academy of Sciences of the United States of America;99;16899-903; 12477932
- Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.;Okazaki Noriko, Kikuno Reiko, Ohara Reiko, Inamoto Susumu, Koseki Haruhiko, Hiraoka Shuichi, Saga Yumiko, Nagase Takahiro, Ohara Osamu, Koga Hisashi, ;2003;DNA research : an international journal for rapid publication of reports on genes and genomes;10;167-80; 14621295
- Protein-protein interaction panel using mouse full-length cDNAs.;Suzuki H, Fukunishi Y, Kagawa I, Saito R, Oda H, Endo T, Kondo S, Bono H, Okazaki Y, Hayashizaki Y, ;2001;Genome research;11;1758-65; 11591653
- Hematopoietic stem cell transplantation without irradiation.;Waskow Claudia, Madan Vikas, Bartels Susanne, Costa Céline, Blasig Rosel, Rodewald Hans-Reimer, ;2009;Nature methods;6;267-9; 19270698