B6.Cg-Tg(CMV-Mapkapk5*L337A)AUlm/Ieg
| Status | Available to order |
| EMMA ID | EM:02114 |
| Citation information | RRID:IMSR_EM:02114 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Tg(CMV-Mapkapk5*L337A)AUlm/Ieg |
| Alternative name | MK5_L337A line A |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(CMV-Mapkapk5*L337A)AUlm |
| Gene/Transgene symbol | Tg(CMV-Mapkapk5*L337A)AUlm |
Information from provider
| Provider | Nancy Gerits |
| Provider affiliation | Medical Biology |
| Genetic information | The construct is a CMV-beta plasmid, with the beta-galactosidase replaced by HA-tagged murine MK5. We mutated this MK5 from Leu337 to Ala. This mutation renders the kinase constitutively active. Random integration. |
| Phenotypic information | No major defects, a very low amount of mice had tumours (2 founder mice), some mice had microbial infections (especially line A was prone). Whilst background breeding, mutated mice seemed more anxious compared to WT, a feature that disappeared the longer the background breeding lasted. |
| Breeding history | The linearized construct was microinjected into CBA x C57BL/6 fertilized eggs. The resulting mice positive for the transgene bred to wt C57BL/6 for 6 generations. |
| References | None available |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |