CD2-Tg(HK2-luc)231Uku/Orl
| Status | Available to order |
| EMMA ID | EM:00217 |
| Citation information | RRID:IMSR_EM:00217 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | CD2-Tg(HK2-luc)231Uku/Orl |
| Alternative name | Uku231 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(HK2-luc)231Uku |
| Gene/Transgene symbol | Tg(HK2-luc)231Uku |
Information from provider
| Provider | Eija Pirinen |
| Provider affiliation | University of Kuopio |
| Genetic information | To generate the transgenic mouse lines, we used constructs containing 254 bp of the human HKII promoter driving 42% of the maximal basal expression of the firefly (P. pyralis) luciferase reporter cDNA in vitro, as described previously by Malkki et al. (1997). For microinjection, DNA fragments encompassing 254 bp of the HKII promoter, 106 bp of HKII exon I noncoding sequence, the luciferase coding sequence (1.8 kb), and small T intron and polyadenylation sequences of SV40 (1.4 kb) were released by digestion with PstI. |
| Phenotypic information | None |
| References |
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Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
Literature references
- Analysis of the human hexokinase II promoter in vivo: lack of insulin response within 4.0 kb.;Pirinen Eija, Heikkinen Sami, Malkki Mari, Deeb Samir S, Jänne Juhani, Laakso Markku, ;2004;Biochimica et biophysica acta;1676;149-54; 14746909