MGI phenotypes (allele matching)
B6.129(SJL)-Kcnk1tm1.1Barh/Orl
| Status | Available to order |
| EMMA ID | EM:02191 |
| Citation information | RRID:IMSR_EM:02191 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129(SJL)-Kcnk1tm1.1Barh/Orl |
| Alternative name | TWIK-1 KO |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Kcnk1tm1.1Barh |
| Gene/Transgene symbol | Kcnk1 |
Information from provider
| Provider | Jacques Barhanin |
| Provider affiliation | IPMC-CNRS |
| Genetic information | cre recombinase-mediated deletion of exon 2 of Kcnk1 gene, which encodes the 'two-pore domains', as well as transmembrane domains TM2 and TM3 of the channel. Sequence analysis of the PCR products showed that deletion of the exon 2 resulted in transcripts encoding a short protein harboring the N and C cytoplasmic ends and the two transmembrane domains TM1 and TM4, separated by a extracellular loop. See ref PubMed ID: 16025300 (Nie, Arrighi et al. 2005). |
| Phenotypic information | Mild hypoglycemia; Renal salt reabsorption defect. |
| Breeding history | Kcnk1 (TWIK-1) +/-; backcrossed to C57BL/6J (Charles River) for 11 generations. Current generation >N11F4. |
| References |
|
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
Disease and phenotype information
Literature references
- Expression and insights on function of potassium channel TWIK-1 in mouse kidney.;Nie Xin, Arrighi Isabelle, Kaissling Brigitte, Pfaff Imke, Mann Jeffrey, Barhanin Jacques, Vallon Volker, ;2005;Pflugers Archiv : European journal of physiology;451;479-88; 16025300
- Adaptive downregulation of a quinidine-sensitive cation conductance in renal principal cells of TWIK-1 knockout mice.;Millar I D, Taylor H C, Cooper G J, Kibble J D, Barhanin J, Robson L, ;2006;Pflugers Archiv : European journal of physiology;453;107-16; 16847696