CD2-Tg(HK2-luc)279Uku/Orl
| Status | Available to order |
| EMMA ID | EM:00220 |
| Citation information | RRID:IMSR_EM:00220 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | CD2-Tg(HK2-luc)279Uku/Orl |
| Alternative name | Uku279 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(HK2-luc)279Uku |
| Gene/Transgene symbol | Tg(HK2-luc)279Uku |
Information from provider
| Provider | Eija Pirinen |
| Provider affiliation | University of Kuopio |
| Genetic information | To generate the transgenic mouse lines, we used construct containing 4077 bp of the human HKII promoter, driving 100% of the maximal basal expression of the firefly (P. pyralis) luciferase reporter cDNA in vitro, as described previously by Malkki et al. (1997). For microinjection, DNA fragments encompassing 4077 bp of the HKII promoter, 106 bp of HKII exon I noncoding sequence, the luciferase coding sequence (1.8 kb), and small T intron and polyadenylation sequences of SV40 (1.4 kb) were released with XhoI after introduction of the XhoI restriction site to replace the upstream PstI site by recloning and ligation of the oligonucleotide linker. The 4077 bp construct contained an internal PstI site. |
| Phenotypic information | None |
| References |
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Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
Literature references
- Analysis of the human hexokinase II promoter in vivo: lack of insulin response within 4.0 kb.;Pirinen Eija, Heikkinen Sami, Malkki Mari, Deeb Samir S, Jänne Juhani, Laakso Markku, ;2004;Biochimica et biophysica acta;1676;149-54; 14746909