B6;D2-Tg(Myh6-ADCY8)7Hj/Orl
| Status | Available to order |
| EMMA ID | EM:02266 |
| Citation information | RRID:IMSR_EM:02266 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;D2-Tg(Myh6-ADCY8)7Hj/Orl |
| Alternative name | HAC8 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Myh6-ADCY8)7Hj |
| Gene/Transgene symbol | Tg(Myh6-ADCY8)7Hj |
Information from provider
| Provider | Nicole Defer |
| Provider affiliation | INSERM U841 |
| Genetic information | Fragment 1956 to 5850 of the human ADCY8 (AC8) cDNA (GeneBank Ac. number:Z35309) was inserted in the Kpn1-EcoRV polylinker restriction site of pGEM(a-MHC) K7 giving the pGEM9z(-)/pGEM-a-MHC-AC8-SV40 recombinant plasmid. Before injection in male pronucleus of one-cell embryo, plasmid was digested with NotI/NsiI and AC8 cDNA purified. |
| Phenotypic information | No apparent phenotype. On basal conditions cardiac function was normal. On release of parasympathetic tone, the intrinsic contractility is heightened and unresponsive to further beta-adrenergic stimulation. Upon aging homozygous animals present higher amount of cardiac fibrosis than wild-type and heterozygous mice. |
| Breeding history | Founders were obtained on 02/1998 and backcrossed with C57BL/6 mice. 3 generations of backcrosses were performed before mating brothers x sisters for homozygotes. Homozygous mice were obtained in 06/2000. All the mice derive from one couple (15 generations). They are currently bred as homozygotes. Homozygous mice are fertile and present no phenotype in basal conditions. |
| References |
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Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
Literature references
- Enhanced cardiac function in transgenic mice expressing a Ca(2+)-stimulated adenylyl cyclase.;Lipskaia L, Defer N, Esposito G, Hajar I, Garel M C, Rockman H A, Hanoune J, ;2000;Circulation research;86;795-801; 10764414
- Augmentation of cardiac contractility with no change in L-type Ca2+ current in transgenic mice with a cardiac-directed expression of the human adenylyl cyclase type 8 (AC8).;Georget Marie, Mateo Philippe, Vandecasteele Grégoire, Jurevicius Jonas, Lipskaia Larissa, Defer Nicole, Hanoune Jacques, Hoerter Jacqueline, Fischmeister Rodolphe, ;2002;FASEB journal : official publication of the Federation of American Societies for Experimental Biology;16;1636-8; 12206999
- Cyclic AMP compartmentation due to increased cAMP-phosphodiesterase activity in transgenic mice with a cardiac-directed expression of the human adenylyl cyclase type 8 (AC8).;Georget Marie, Mateo Philippe, Vandecasteele Grégoire, Lipskaia Larissa, Defer Nicole, Hanoune Jacques, Hoerter Jacqueline, Lugnier Claire, Fischmeister Rodolphe, ;2003;FASEB journal : official publication of the Federation of American Societies for Experimental Biology;17;1380-91; 12890691