B6.129-Penk-rs^tm1Pig/Ieg

Status	Available to order
EMMA ID	EM:02270
Citation information	RRID:IMSR_EM:02270 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.129-Penk-rs^tm1Pig/Ieg
Alternative name	Penk tm1zim
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Penk-rs^tm1Pig
Gene/Transgene symbol	Penk-rs

Information from provider

Provider	Andreas Zimmer
Provider affiliation	Institute of Molecular Psychiatry
Genetic information	See genetic description in König M. et al. "Pain responses, anxiety and aggression in mice deficient in pre-proenkephalin" Nature 383(6600):535-8 (1996), PMID 8849726.
Phenotypic information	Hyperalgesia, increased anxiety, aggressiveness.
Breeding history	Backcrossed for at least 10 generations to C57BL/6J mice.
References	Region selective up-regulation of micro-, delta- and kappa-opioid receptors but not opioid receptor-like 1 receptors in the brains of enkephalin and dynorphin knockout mice.;Clarke S, Zimmer A, Zimmer A M, Hill R G, Kitchen I, ;2003;Neuroscience;122;479-89; 14614912 Preproenkephalin knockout mice show no depression-related phenotype.;Bilkei-Gorzo Andras, Michel Kerstin, Noble Florence, Roques Bernard P, Zimmer Andreas, ;2007;Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology;32;2330-7; 17375141 Nicotine-induced antinociception, rewarding effects, and physical dependence are decreased in mice lacking the preproenkephalin gene.;Berrendero Fernando, Mendizábal Victoria, Robledo Patricia, Galeote Lola, Bilkei-Gorzo Andras, Zimmer Andreas, Maldonado Rafael, ;2005;The Journal of neuroscience : the official journal of the Society for Neuroscience;25;1103-12; 15689546 Quantitative autoradiography of adenosine receptors and NBTI-sensitive adenosine transporters in the brains of mice deficient in the preproenkephalin gene.;Bailey Alexis, Weber Daniel, Zimmer A, Zimmer A M, Hourani Susanna M O, Kitchen Ian, ;2004;Brain research;1025;1-9; 15464738 Expression of the Alzheimer protease BACE1 is suppressed via its 5'-untranslated region.;Lammich Sven, Schöbel Susanne, Zimmer Ann-Katrin, Lichtenthaler Stefan F, Haass Christian, ;2004;EMBO reports;5;620-5; 15167888 Enkephalin release and opioid receptor activation does not mediate the antinociceptive or sedative/hypnotic effects of nitrous oxide.;Kingery W S, Sawamura S, Agashe G S, Davies M F, Clark J D, Zimmer A, ;2001;European journal of pharmacology;427;27-35; 11553360 Th2-dependent airway eosinophilia is regulated by preproenkephalin.;Hook S, Prout M, Camberis M, Konig M, Zimmer A, Van Heeke G, Le Gros G, ;2000;Journal of neuroimmunology;107;59-65; 10808051 Cannabinoid withdrawal syndrome is reduced in pre-proenkephalin knock-out mice.;Valverde O, Maldonado R, Valjent E, Zimmer A M, Zimmer A, ;2000;The Journal of neuroscience : the official journal of the Society for Neuroscience;20;9284-9; 11125007 Preproenkephalin is a Th2 cytokine but is not required for Th2 differentiation in vitro.;Hook S, Camberis M, Prout M, Konig M, Zimmer A, Van Heeke G, Le Gros G, ;1999;Immunology and cell biology;77;385-90; 10540203 Region-specific up-regulation of opioid receptor binding in enkephalin knockout mice.;Brady L S, Herkenham M, Rothman R B, Partilla J S, König M, Zimmer A M, Zimmer A, ;1999;Brain research. Molecular brain research;68;193-7; 10320798 Pain responses, anxiety and aggression in mice deficient in pre-proenkephalin.;König M, Zimmer A M, Steiner H, Holmes P V, Crawley J N, Brownstein M J, Zimmer A, ;1996;Nature;383;535-8; 8849726

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

MGI phenotypes (allele matching)

decreased chemically-elicited antinociception / MGI
abnormal associative learning / MGI
behavior/neurological phenotype / MGI
decreased physiological sensitivity to xenobiotic / MGI
impaired behavioral response to nicotine / MGI
decreased behavioral withdrawal response / MGI
lung inflammation / MGI
abnormal T-helper 2 physiology / MGI
decreased T-helper 2 cell number / MGI
increased aggression towards mice / MGI
increased aggression towards humans / MGI
increased anxiety-related response / MGI
decreased exploration in new environment / MGI
abnormal touch/ nociception / MGI
abnormal emotion/affect behavior / MGI
abnormal chemical nociception / MGI
increased thigmotaxis / MGI
decreased thermal nociceptive threshold / MGI
reproductive system phenotype / MGI

MGI phenotypes (gene matching)

increased aggression towards mice / MGI
increased aggression towards humans / MGI
increased anxiety-related response / MGI
decreased exploration in new environment / MGI
lung inflammation / MGI
abnormal touch/ nociception / MGI
decreased chemically-elicited antinociception / MGI
abnormal associative learning / MGI
abnormal emotion/affect behavior / MGI
abnormal chemical nociception / MGI
increased thigmotaxis / MGI
decreased thermal nociceptive threshold / MGI
behavior/neurological phenotype / MGI
reproductive system phenotype / MGI
abnormal T-helper 2 physiology / MGI
decreased T-helper 2 cell number / MGI
decreased physiological sensitivity to xenobiotic / MGI
impaired behavioral response to nicotine / MGI
decreased behavioral withdrawal response / MGI

Literature references

Region selective up-regulation of micro-, delta- and kappa-opioid receptors but not opioid receptor-like 1 receptors in the brains of enkephalin and dynorphin knockout mice.;Clarke S, Zimmer A, Zimmer A M, Hill R G, Kitchen I, ;2003;Neuroscience;122;479-89; 14614912
Preproenkephalin knockout mice show no depression-related phenotype.;Bilkei-Gorzo Andras, Michel Kerstin, Noble Florence, Roques Bernard P, Zimmer Andreas, ;2007;Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology;32;2330-7; 17375141
Nicotine-induced antinociception, rewarding effects, and physical dependence are decreased in mice lacking the preproenkephalin gene.;Berrendero Fernando, Mendizábal Victoria, Robledo Patricia, Galeote Lola, Bilkei-Gorzo Andras, Zimmer Andreas, Maldonado Rafael, ;2005;The Journal of neuroscience : the official journal of the Society for Neuroscience;25;1103-12; 15689546
Quantitative autoradiography of adenosine receptors and NBTI-sensitive adenosine transporters in the brains of mice deficient in the preproenkephalin gene.;Bailey Alexis, Weber Daniel, Zimmer A, Zimmer A M, Hourani Susanna M O, Kitchen Ian, ;2004;Brain research;1025;1-9; 15464738
Expression of the Alzheimer protease BACE1 is suppressed via its 5'-untranslated region.;Lammich Sven, Schöbel Susanne, Zimmer Ann-Katrin, Lichtenthaler Stefan F, Haass Christian, ;2004;EMBO reports;5;620-5; 15167888
Enkephalin release and opioid receptor activation does not mediate the antinociceptive or sedative/hypnotic effects of nitrous oxide.;Kingery W S, Sawamura S, Agashe G S, Davies M F, Clark J D, Zimmer A, ;2001;European journal of pharmacology;427;27-35; 11553360
Th2-dependent airway eosinophilia is regulated by preproenkephalin.;Hook S, Prout M, Camberis M, Konig M, Zimmer A, Van Heeke G, Le Gros G, ;2000;Journal of neuroimmunology;107;59-65; 10808051
Cannabinoid withdrawal syndrome is reduced in pre-proenkephalin knock-out mice.;Valverde O, Maldonado R, Valjent E, Zimmer A M, Zimmer A, ;2000;The Journal of neuroscience : the official journal of the Society for Neuroscience;20;9284-9; 11125007
Preproenkephalin is a Th2 cytokine but is not required for Th2 differentiation in vitro.;Hook S, Camberis M, Prout M, Konig M, Zimmer A, Van Heeke G, Le Gros G, ;1999;Immunology and cell biology;77;385-90; 10540203
Region-specific up-regulation of opioid receptor binding in enkephalin knockout mice.;Brady L S, Herkenham M, Rothman R B, Partilla J S, König M, Zimmer A M, Zimmer A, ;1999;Brain research. Molecular brain research;68;193-7; 10320798
Pain responses, anxiety and aggression in mice deficient in pre-proenkephalin.;König M, Zimmer A M, Steiner H, Holmes P V, Crawley J N, Brownstein M J, Zimmer A, ;1996;Nature;383;535-8; 8849726
Pre-proenkephalin 1 is Downregulated Under Unloading and is Involved in Osteoblast Biology.;Puri Chiara, Dannenberg Charlotte, Ucci Argia, Ponzetti Marco, Pucci Elisa, Silvestri Luciana, Lau Patrick, Frings-Meuthen Petra, Heer Martina, Rucci Nadia, Teti Anna, Maurizi Antonio, ;2024;Calcified tissue international;114;524-534; 38506955

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Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*

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Practical information

Genotyping protocol

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B6.129-Penk-rstm1Pig/Ieg