B6.129P2-Scn10a^tm1Jwo/JwoH

Status	Available to order
EMMA ID	EM:02290
Citation information	RRID:IMSR_EM:02290 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.129P2-Scn10a^tm1Jwo/JwoH
Alternative name	Nav1.8 knockout mouse
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Scn10a^tm1Jwo
Gene/Transgene symbol	Scn10a

Information from provider

Provider	John Wood
Provider affiliation	University College Lonodon
Genetic information	A lacZ and neomycin cassette was inserted in place of exon 4 and 5, plus insertion of stop codons.
Phenotypic information	Defects in mechanical and inflammatory pain tests.
Breeding history	Backcrossed for more than 50 generations to C57BL/6.
References	The tetrodotoxin-resistant sodium channel SNS has a specialized function in pain pathways.;Akopian A N, Souslova V, England S, Okuse K, Ogata N, Ure J, Smith A, Kerr B J, McMahon S B, Boyce S, Hill R, Stanfa L C, Dickenson A H, Wood J N, ;1999;Nature neuroscience;2;541-8; 10448219 A role for the TTX-resistant sodium channel Nav 1.8 in NGF-induced hyperalgesia, but not neuropathic pain.;Kerr B J, Souslova V, McMahon S B, Wood J N, ;2001;Neuroreport;12;3077-80; 11568640 Sensory neuron sodium channel Nav1.8 is essential for pain at low temperatures.;Zimmermann Katharina, Leffler Andreas, Babes Alexandru, Cendan Cruz Miguel, Carr Richard W, Kobayashi Jin-ichi, Nau Carla, Wood John N, Reeh Peter W, ;2007;Nature;447;855-8; 17568746

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	homozygous C57BL/6 males
Breeding at archiving centre	Males were archived by sperm freezing on arrival. No breeding was performed at the archiving centre.

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Romano-Ward syndrome / Orphanet_101016
Sodium channelopathy-related small fiber neuropathy / Orphanet_306577

MGI phenotypes (gene matching)

abnormal tail movements / MGI
abnormal posture / MGI
impaired righting response / MGI
apnea / MGI
abnormal pain threshold / MGI
abnormal nervous system electrophysiology / MGI
impaired ability to fire action potentials / MGI
abnormal thermal nociception / MGI
abnormal mechanical nociception / MGI
catalepsy / MGI
hypoalgesia / MGI
abnormal single cell response / MGI
decreased thermal nociceptive threshold / MGI
abnormal brain wave pattern / MGI
behavior/neurological phenotype / MGI
abnormal action potential / MGI
hyperalgesia / MGI
abnormal RR interval / MGI
sinus bradycardia / MGI

Literature references

The tetrodotoxin-resistant sodium channel SNS has a specialized function in pain pathways.;Akopian A N, Souslova V, England S, Okuse K, Ogata N, Ure J, Smith A, Kerr B J, McMahon S B, Boyce S, Hill R, Stanfa L C, Dickenson A H, Wood J N, ;1999;Nature neuroscience;2;541-8; 10448219
A role for the TTX-resistant sodium channel Nav 1.8 in NGF-induced hyperalgesia, but not neuropathic pain.;Kerr B J, Souslova V, McMahon S B, Wood J N, ;2001;Neuroreport;12;3077-80; 11568640
Sensory neuron sodium channel Nav1.8 is essential for pain at low temperatures.;Zimmermann Katharina, Leffler Andreas, Babes Alexandru, Cendan Cruz Miguel, Carr Richard W, Kobayashi Jin-ichi, Nau Carla, Wood John N, Reeh Peter W, ;2007;Nature;447;855-8; 17568746

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Example health report
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Material Transfer Agreement (MTA)
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B6.129P2-Scn10atm1Jwo/JwoH