B6;CBA-Tg(Igf2/LacZ,H19)YZ15Aco/AcoH

Status	Available to order
EMMA ID	EM:02380
Citation information	RRID:IMSR_EM:02380 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6;CBA-Tg(Igf2/LacZ,H19)YZ15Aco/AcoH
Alternative name	YZ15
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Igf2/LacZ,H19)YZ15Aco
Gene/Transgene symbol	Tg(Igf2/LacZ,H19)YZ15Aco

Information from provider

Provider	Justin Ainscough
Provider affiliation	Central Biomedical Services, University of Leeds
Genetic information	The YZ15 line (ten copy transgene) contains an additional 130 kb genomic region from mouse distal chromosome 7 spanning from Igf2 to ~40 kb downstream of H19. Igf2 has been inactivated by insertional mutagenesis resulting in an Igf2-LacZ gene fusion. LacZ activity shows spatial expression of Igf2 in a subset of tissues. The imprinted H19 gene is only expressed when transmitted through the maternal germline. After paternal transmission the gene is repressed. Unlike low copy number lines, the Igf2-LacZ gene does not show appropriate reciprocal imprinting in YZ15 mice as it is expressed from both parental alleles.
Phenotypic information	The line has not been kept homozygous due to reduced fertility.
Breeding history	Brother sister matings with sporadic backcrossing to C57BL/6.
References	Imprinting of Igf2 and H19 from a 130 kb YAC transgene.;Ainscough J F, Koide T, Tada M, Barton S, Surani M A, ;1997;Development (Cambridge, England);124;3621-32; 9342054 Appropriate expression of the mouse H19 gene utilises three or more distinct enhancer regions spread over more than 130 kb.;Ainscough J F, Dandolo L, Surani M A, ;2000;Mechanisms of development;91;365-8; 10704866

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Literature references

Imprinting of Igf2 and H19 from a 130 kb YAC transgene.;Ainscough J F, Koide T, Tada M, Barton S, Surani M A, ;1997;Development (Cambridge, England);124;3621-32; 9342054
Appropriate expression of the mouse H19 gene utilises three or more distinct enhancer regions spread over more than 130 kb.;Ainscough J F, Dandolo L, Surani M A, ;2000;Mechanisms of development;91;365-8; 10704866

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