B6.129P2-Slc38a4tm2Kel/KelH
| Status | Available to order |
| EMMA ID | EM:02390 |
| Citation information | RRID:IMSR_EM:02390 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2-Slc38a4tm2Kel/KelH |
| Alternative name | Slc38a4tm2Kel/DeltaCG1A |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Slc38a4tm2Kel |
| Gene/Transgene symbol | Slc38a4 |
Information from provider
| Provider | Gavin Kelsey |
| Provider affiliation | The Babraham Institute, Babraham, Cambridge, CB2 4PB |
| Genetic information | A loxP-flanked cassette containing neo:URA:tACE-Cre replaced the region of maternal-germline derived differential methylation, which is the putative imprinting control region for the imprinting of the Slc38a4 gene. The neo:URA:tACE-Cre cassette was excised on passage of the allele through the male germline, leaving an allele in which the differentially methylated region was deleted and replaced by a loxP site. |
| Phenotypic information | None. A minor effect on term weight. |
| Breeding history | Germline transmitting chimaeras for the targeted allele were crossed with C57BL/6JOlaHsd to produce (C57BL/6JOlaHsd x 129P2/Ola)F1. F1 animals were then backcrossed with C57BL/6JOlaHsd and backcrossing to C57BL/6JOlaHsd has continued over 8 generations. The targeted allele has always been maintained in a heterozygous state. |
| References |
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Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Identification of novel imprinted genes in a genome-wide screen for maternal methylation.;Smith Rachel J, Dean Wendy, Konfortova Galia, Kelsey Gavin, ;2003;Genome research;13;558-69; 12670997