B6.Cg-Tg(TP1-nlacZ)1Cota/Orl

Status	Available to order
EMMA ID	EM:02412
Citation information	RRID:IMSR_EM:02412 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.Cg-Tg(TP1-nlacZ)1Cota/Orl
Alternative name	NAS
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(TP1-nlacZ)1Cota
Gene/Transgene symbol	Tg(TP1-nlacZ)1Cota

Information from provider

Provider	Michel Cohen-Tannoudji
Provider affiliation	Departement de Biologie du Développement, Unite de Génétique Fonctionnelle de la Souris, URA 2578 CNRS/Institut Pasteur
Genetic information	RBP-J, a DNA binding protein, is the effector of Notch activity in the nucleus and is used as such by the four mammalian Notch receptors. We have produced the TP1-nlacZ construct consisting of a nls-lacZ reporter gene under the control of a minimal promoter and multiple RBP-J binding sites. Activity of this transgene is therefore expected to monitor the activity transduced by all 4 Notch receptors. The NAS transgenic mouse line has been derived by microinjection of the Tp1-nlacZ construct.
Phenotypic information	none
Breeding history	10 backcrosses have been performed on C57BL/6.
References	Nas transgenic mouse line allows visualization of Notch pathway activity in vivo.;Souilhol Céline, Cormier Sarah, Monet Marie, Vandormael-Pournin Sandrine, Joutel Anne, Babinet Charles, Cohen-Tannoudji Michel, ;2006;Genesis (New York, N.Y. : 2000);44;277-86; 16708386 The archetypal R90C CADASIL-NOTCH3 mutation retains NOTCH3 function in vivo.;Monet Marie, Domenga Valérie, Lemaire Barbara, Souilhol Céline, Langa Francina, Babinet Charles, Gridley Thomas, Tournier-Lasserve Elisabeth, Cohen-Tannoudji Michel, Joutel Anne, ;2007;Human molecular genetics;16;982-92; 17331978

Information from EMMA

Archiving centre	CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France

Literature references

Nas transgenic mouse line allows visualization of Notch pathway activity in vivo.;Souilhol Céline, Cormier Sarah, Monet Marie, Vandormael-Pournin Sandrine, Joutel Anne, Babinet Charles, Cohen-Tannoudji Michel, ;2006;Genesis (New York, N.Y. : 2000);44;277-86; 16708386
The archetypal R90C CADASIL-NOTCH3 mutation retains NOTCH3 function in vivo.;Monet Marie, Domenga Valérie, Lemaire Barbara, Souilhol Céline, Langa Francina, Babinet Charles, Gridley Thomas, Tournier-Lasserve Elisabeth, Cohen-Tannoudji Michel, Joutel Anne, ;2007;Human molecular genetics;16;982-92; 17331978

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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