| Status | Available to order |
| EMMA ID | EM:02421 |
| Citation information | RRID:IMSR_EM:02421 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | STOCK Prnptm1Cwe Tg(Prnp/PRNP)1Drb/H |
| Alternative name | PrP Chicken-repeats |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Prnptm1Cwe, Tg(Prnp/PRNP)1Drb |
| Gene/Transgene symbol | Prnp, Tg(Prnp/PRNP)1Drb |
| Provider | David Brown |
| Provider affiliation | University of Bath |
| Genetic information | The octameric repeat region of the mouse prion protein was replaced with the hexameric repeat region from the domestic chicken. The mice express mouse PrP at wild-type levels with correct membrane location and orientation but have an altered metal binding region. The transgene is expressed on a background lacking wild-type PrP expression (PrP knockout). This implies that only the mutant PrP is expressed in these mice. The PrP knockout mice used for the crosses were those described in Bueler et al., 1992. |
| Phenotypic information | None |
| References | None available |
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
| Animals used for archiving | homozygous 129/Sv (synonym: 129Sv) males, homozygous 129/Sv (synonym: 129Sv) females |
| Breeding at archiving centre | A colony of double homozygous mice was established at the archiving centre by intercrossing imported individuals. Archiving as embryos was performed by IVF (using double homozygous sperm and oocyte donors) and by natural mating. |
| Stage of embryos | 2-cell |
Orphanet associated rare diseases, based on orthologous gene matching
