B6.129P2-Wt1tm1Hst/H

Status

Available to order

EMMA IDEM:02465
Citation informationRRID:IMSR_EM:02465 

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International strain nameB6.129P2-Wt1tm1Hst/H
Alternative nameWT*
Strain typeTargeted Mutant Strains : Point mutation
Allele/Transgene symbolWt1tm1Hst
Gene/Transgene symbolWt1

Information from provider

ProviderColin Miles
Provider affiliationInstitute of Human Genetics, Newcastle University
Additional ownerProf. N.D. Hastie, MRC Human Genetics Unit, Edinburgh, UK.
Genetic informationTargeted introduction of STOP codon to ablate Wt1 isoforms originating from the alternative, upstream CTG translation initiator (WT* isoforms).
Phenotypic informationNo detectable WT* protein isoforms by Western blot. No overt phenotype.
Breeding historyBackcrossed to C57BL/6, more than 10 generations. Currently maintained as homozygous stock.
References
  • Mice lacking the 68-amino-acid, mammal-specific N-terminal extension of WT1 develop normally and are fertile.;Miles Colin G, Slight Joan, Spraggon Lee, O'Sullivan Maureen, Patek Charles, Hastie Nicholas D, ;2003;Molecular and cellular biology;23;2608-13; 12640141
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisednot known

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivingheterozygous C57BL/6 males
Breeding at archiving centreNone

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (gene matching)
  • abnormal heart development / MGI
  • abnormal heart shape / MGI
  • kidney cortex cysts / MGI
  • abnormal kidney development / MGI
  • abnormal kidney blood vessel morphology / MGI
  • abnormal liver development / MGI
  • liver hypoplasia / MGI
  • absent spleen / MGI
  • small testis / MGI
  • abnormal lung development / MGI
  • pulmonary hypoplasia / MGI
  • decreased embryo size / MGI
  • edema / MGI
  • abnormal reproductive system physiology / MGI
  • male infertility / MGI
  • prenatal lethality / MGI
  • premature death / MGI
  • abnormal embryonic tissue morphology / MGI
  • no abnormal phenotype detected / MGI
  • small heart / MGI
  • decreased germ cell number / MGI
  • abnormal secondary sex determination / MGI
  • streak gonad / MGI
  • dilated renal tubules / MGI
  • glomerulonephritis / MGI
  • increased urine protein level / MGI
  • small kidney / MGI
  • primary sex reversal / MGI
  • renal interstitial fibrosis / MGI
  • absent ovary / MGI
  • kidney failure / MGI
  • kidney cysts / MGI
  • diaphragmatic hernia / MGI
  • azoospermia / MGI
  • hemopericardium / MGI
  • glomerulosclerosis / MGI
  • absent adrenal gland / MGI
  • abnormal renal glomerulus morphology / MGI
  • abnormal podocyte morphology / MGI
  • abnormal mesangial cell morphology / MGI
  • renal/urinary system phenotype / MGI
  • cardiovascular system phenotype / MGI
  • agonadal / MGI
  • abnormal mesenchyme morphology / MGI
  • absent testes / MGI
  • absent podocyte foot process / MGI
  • podocyte foot process effacement / MGI
  • increased splenocyte apoptosis / MGI
  • abnormal spleen development / MGI
  • myocardium hypoplasia / MGI
  • primary atelectasis / MGI
  • neonatal lethality, complete penetrance / MGI
  • perinatal lethality, complete penetrance / MGI
  • embryonic lethality, complete penetrance / MGI
  • lethality throughout fetal growth and development, complete penetrance / MGI
  • prenatal lethality, incomplete penetrance / MGI
  • embryonic lethality during organogenesis, incomplete penetrance / MGI
  • juxtaglomerular cell hyperplasia / MGI
  • abnormal kidney interlobular artery morphology / MGI
  • abnormal glomerular capillary morphology / MGI
  • expanded mesangial matrix / MGI
  • absent metanephros / MGI
  • increased metanephric mesenchyme apoptosis / MGI
  • renal cast / MGI
  • increased renal glomerulus basement membrane thickness / MGI
  • abnormal gonadal ridge morphology / MGI
  • mesangial cell hyperplasia / MGI
  • mesangiolysis / MGI
  • abnormal glomerular capsule parietal layer morphology / MGI
  • decreased glomerular capsule space / MGI
  • glomerular crescent / MGI
  • podocyte microvillus transformation / MGI
  • podocyte hypertrophy / MGI
  • abnormal diaphragm development / MGI
  • abnormal pleuropericardial membrane morphology / MGI
  • abnormal heart apex morphology / MGI
  • abnormal adrenal gland development / MGI

Literature references

  • Mice lacking the 68-amino-acid, mammal-specific N-terminal extension of WT1 develop normally and are fertile.;Miles Colin G, Slight Joan, Spraggon Lee, O'Sullivan Maureen, Patek Charles, Hastie Nicholas D, ;2003;Molecular and cellular biology;23;2608-13; 12640141

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Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
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