B6.129P2-Sema3eGt(RST629)Byg/Ieg

Status

Available to order

EMMA IDEM:02473
Citation informationRRID:IMSR_EM:02473 

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International strain nameB6.129P2-Sema3eGt(RST629)Byg/Ieg
Alternative nameRST629
Strain typeGene-trap
Allele/Transgene symbolSema3eGt(RST629)Byg
Gene/Transgene symbolSema3e

Information from provider

ProviderRoland Friedel
Provider affiliationSoriano Lab, Department of Developomental & Regenerative Biology, Mount Sinai School of Medicine
Additional ownerDr. Tessier-Lavigne, Genentech Inc, San Francisco CA, USA
Genetic informationThe allele RST629 represents an insertion of a gene trap vector (pGT0TMpfs) into the fourth intron of the Semaphorin3E gene. The trap vector contains lacZ and PLAP reporters. Northern blotting has shown that the mutation completely disrupts the wildtype mRNA.
Phenotypic informationHomozygous mutants are viable and fertile. No gross abnormalities have been detected.
Breeding historyBackcrossed for 5 generations to C57BL/6.
References
  • Defining brain wiring patterns and mechanisms through gene trapping in mice.;Leighton P A, Mitchell K J, Goodrich L V, Lu X, Pinson K, Scherz P, Skarnes W C, Tessier-Lavigne M, ;2001;Nature;410;174-9; 11242070
  • Functional analysis of secreted and transmembrane proteins critical to mouse development.;Mitchell K J, Pinson K I, Kelly O G, Brennan J, Zupicich J, Scherz P, Leighton P A, Goodrich L V, Lu X, Avery B J, Tate P, Dill K, Pangilinan E, Wakenight P, Tessier-Lavigne M, Skarnes W C, ;2001;Nature genetics;28;241-9; 11431694
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous C57BL/6J males, wild-type C57BL/6J females
Stage of embryos2-cell

Disease and phenotype information

IMPC phenotypes (gene matching)
  • persistence of hyaloid vascular system / IMPC
  • irregularly shaped pupil / IMPC
  • decreased circulating phosphate level / IMPC
  • impaired pupillary reflex / IMPC
  • abnormal placement of pupils / IMPC
  • abnormal retina blood vessel morphology / IMPC
  • abnormal retina morphology / IMPC
  • abnormal locomotor behavior / IMPC
MGI phenotypes (gene matching)
  • abnormal motor neuron morphology / MGI
  • abnormal motor neuron innervation pattern / MGI
  • abnormal retina morphology / MGI
  • abnormal somite development / MGI
  • abnormal retinal vasculature morphology / MGI
  • nervous system phenotype / MGI
  • behavior/neurological phenotype / MGI
  • abnormal intersomitic vessel morphology / MGI
  • Bergmeister's papilla / MGI

Literature references

  • Defining brain wiring patterns and mechanisms through gene trapping in mice.;Leighton P A, Mitchell K J, Goodrich L V, Lu X, Pinson K, Scherz P, Skarnes W C, Tessier-Lavigne M, ;2001;Nature;410;174-9; 11242070
  • Functional analysis of secreted and transmembrane proteins critical to mouse development.;Mitchell K J, Pinson K I, Kelly O G, Brennan J, Zupicich J, Scherz P, Leighton P A, Goodrich L V, Lu X, Avery B J, Tate P, Dill K, Pangilinan E, Wakenight P, Tessier-Lavigne M, Skarnes W C, ;2001;Nature genetics;28;241-9; 11431694

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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