B6.129P2-Sema6a^{Gt(KST069)Byg}/Ieg

Status	Available to order
EMMA ID	EM:02480
Citation information	RRID:IMSR_EM:02480 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.129P2-Sema6a^{Gt(KST069)Byg}/Ieg
Alternative name	KST69
Strain type	Gene-trap
Allele/Transgene symbol	Sema6a^{Gt(KST069)Byg}
Gene/Transgene symbol	Sema6a

Information from provider

Provider	Roland Friedel
Provider affiliation	Soriano Lab, Department of Developomental & Regenerative Biology, Mount Sinai School of Medicine
Additional owner	Dr. Kevin Mitchell, Trinity College Dublin, Smurfit Institute of Genetics, Dublin, Ireland
Genetic information	Official MGI allele name: Sema6Gt(pGT1TM)69Wcs. Short name: KST69. The allele KST69 is an insertion of a gene trap vector (pGT1TMpfs) into the intron that disrupts amino acid 473. This results in the complete abolition of wildtype transcript. The trap vector contains lacZ and PLAP reporters.
Phenotypic information	Homozygous mutants are viable. Homozygous mutants reveal misprojections of axons in the forebrain and a migration defect of granule cells in the cerebellum.
Breeding history	Backcrossed for 16 generations to C57BL/6.
References	Defining brain wiring patterns and mechanisms through gene trapping in mice.;Leighton P A, Mitchell K J, Goodrich L V, Lu X, Pinson K, Scherz P, Skarnes W C, Tessier-Lavigne M, ;2001;Nature;410;174-9; 11242070 Functional analysis of secreted and transmembrane proteins critical to mouse development.;Mitchell K J, Pinson K I, Kelly O G, Brennan J, Zupicich J, Scherz P, Leighton P A, Goodrich L V, Lu X, Avery B J, Tate P, Dill K, Pangilinan E, Wakenight P, Tessier-Lavigne M, Skarnes W C, ;2001;Nature genetics;28;241-9; 11431694 Plexin-A2 and its ligand, Sema6A, control nucleus-centrosome coupling in migrating granule cells.;Renaud Julie, Kerjan Géraldine, Sumita Itsuko, Zagar Yvrick, Georget Virginie, Kim Doyeun, Fouquet Coralie, Suda Kazunori, Sanbo Makoto, Suto Fumikazu, Ackerman Susan L, Mitchell Kevin J, Fujisawa Hajime, Chédotal Alain, ;2008;Nature neuroscience;11;440-9; 18327254 The transmembrane semaphorin Sema6A controls cerebellar granule cell migration.;Kerjan Géraldine, Dolan Jackie, Haumaitre Cécile, Schneider-Maunoury Sylvie, Fujisawa Hajime, Mitchell Kevin J, Chédotal Alain, ;2005;Nature neuroscience;8;1516-24; 16205717 Interactions between plexin-A2, plexin-A4, and semaphorin 6A control lamina-restricted projection of hippocampal mossy fibers.;Suto Fumikazu, Tsuboi Miu, Kamiya Haruyuki, Mizuno Hidenobu, Kiyama Yuji, Komai Shoji, Shimizu Masayuki, Sanbo Makoto, Yagi Takeshi, Hiromi Yasushi, Chédotal Alain, Mitchell Kevin J, Manabe Toshiya, Fujisawa Hajime, ;2007;Neuron;53;535-47; 17296555
Homozygous fertile	not known
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6J males, wild-type C57BL/6J females
Stage of embryos	2-cell

Disease and phenotype information

MGI phenotypes (allele matching)

abnormal hippocampal mossy fiber morphology / MGI
nervous system phenotype / MGI
abnormal retinal inner plexiform layer morphology / MGI
abnormal amacrine cell morphology / MGI
abnormal retinal ganglion cell morphology / MGI
abnormal dendrite morphology / MGI
abnormal neurite morphology / MGI
abnormal forebrain morphology / MGI
behavior/neurological phenotype / MGI
reproductive system phenotype / MGI

MGI phenotypes (gene matching)

abnormal forebrain morphology / MGI
abnormal hippocampal mossy fiber morphology / MGI
nervous system phenotype / MGI
abnormal retinal inner plexiform layer morphology / MGI
abnormal amacrine cell morphology / MGI
behavior/neurological phenotype / MGI
reproductive system phenotype / MGI
abnormal retinal ganglion cell morphology / MGI
abnormal dendrite morphology / MGI
abnormal neurite morphology / MGI

Literature references

Defining brain wiring patterns and mechanisms through gene trapping in mice.;Leighton P A, Mitchell K J, Goodrich L V, Lu X, Pinson K, Scherz P, Skarnes W C, Tessier-Lavigne M, ;2001;Nature;410;174-9; 11242070
Functional analysis of secreted and transmembrane proteins critical to mouse development.;Mitchell K J, Pinson K I, Kelly O G, Brennan J, Zupicich J, Scherz P, Leighton P A, Goodrich L V, Lu X, Avery B J, Tate P, Dill K, Pangilinan E, Wakenight P, Tessier-Lavigne M, Skarnes W C, ;2001;Nature genetics;28;241-9; 11431694
Plexin-A2 and its ligand, Sema6A, control nucleus-centrosome coupling in migrating granule cells.;Renaud Julie, Kerjan Géraldine, Sumita Itsuko, Zagar Yvrick, Georget Virginie, Kim Doyeun, Fouquet Coralie, Suda Kazunori, Sanbo Makoto, Suto Fumikazu, Ackerman Susan L, Mitchell Kevin J, Fujisawa Hajime, Chédotal Alain, ;2008;Nature neuroscience;11;440-9; 18327254
The transmembrane semaphorin Sema6A controls cerebellar granule cell migration.;Kerjan Géraldine, Dolan Jackie, Haumaitre Cécile, Schneider-Maunoury Sylvie, Fujisawa Hajime, Mitchell Kevin J, Chédotal Alain, ;2005;Nature neuroscience;8;1516-24; 16205717
Interactions between plexin-A2, plexin-A4, and semaphorin 6A control lamina-restricted projection of hippocampal mossy fibers.;Suto Fumikazu, Tsuboi Miu, Kamiya Haruyuki, Mizuno Hidenobu, Kiyama Yuji, Komai Shoji, Shimizu Masayuki, Sanbo Makoto, Yagi Takeshi, Hiromi Yasushi, Chédotal Alain, Mitchell Kevin J, Manabe Toshiya, Fujisawa Hajime, ;2007;Neuron;53;535-47; 17296555

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6.129P2-Sema6aGt(KST069)Byg/Ieg