C3HeB/FeJ-Tent5a^m1Mhda/Ieg

Status	Available to order
EMMA ID	EM:02545
Citation information	RRID:IMSR_EM:02545 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C3HeB/FeJ-Tent5a^m1Mhda/Ieg
Alternative name	BAP014
Strain type	Induced Mutant Strains : Chemically-induced
Allele/Transgene symbol	Tent5a^m1Mhda
Gene/Transgene symbol	Tent5a

Information from provider

Provider	Martin Hrabe de Angelis
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	Mutation: Fam46a c.469 G-to-T, p.E157*
Phenotypic information	Alkaline phosphatase high
Breeding history	More than 2 times backcrossed to C3HeB/FeJ
References	Genome-wide, large-scale production of mutant mice by ENU mutagenesis.;Hrabé de Angelis M H, Flaswinkel H, Fuchs H, Rathkolb B, Soewarto D, Marschall S, Heffner S, Pargent W, Wuensch K, Jung M, Reis A, Richter T, Alessandrini F, Jakob T, Fuchs E, Kolb H, Kremmer E, Schaeble K, Rollinski B, Roscher A, Peters C, Meitinger T, Strom T, Steckler T, Holsboer F, Klopstock T, Gekeler F, Schindewolf C, Jung T, Avraham K, Behrendt H, Ring J, Zimmer A, Schughart K, Pfeffer K, Wolf E, Balling R, ;2000;Nature genetics;25;444-7; 10932192 Screening for dysmorphological abnormalities--a powerful tool to isolate new mouse mutants.;Fuchs H, Schughart K, Wolf E, Balling R, Hrabé de Angelis M, ;2000;Mammalian genome : official journal of the International Mammalian Genome Society;11;528-30; 10886017 Exome sequencing identifies a nonsense mutation in Fam46a associated with bone abnormalities in a new mouse model for skeletal dysplasia.;Diener Susanne, Bayer Sieglinde, Sabrautzki Sibylle, Wieland Thomas, Mentrup Birgit, Przemeck Gerhard K H, Rathkolb Birgit, Graf Elisabeth, Hans Wolfgang, Fuchs Helmut, Horsch Marion, Schwarzmayr Thomas, Wolf Eckhard, Klopocki Eva, Jakob Franz, Strom Tim M, Hrabě de Angelis Martin, Lorenz-Depiereux Bettina, ;2016;Mammalian genome : official journal of the International Mammalian Genome Society;27;111-21; 26803617
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C3H males

Disease and phenotype information

IMPC phenotypes (gene matching)

decreased Ly6C-positive mature NK cell number / IMPC
abnormal bone structure / IMPC
abnormal ulna morphology / IMPC
abnormal tibia morphology / IMPC
abnormal tooth morphology / IMPC
abnormal cranium morphology / IMPC
impaired righting response / IMPC
abnormal joint morphology / IMPC
decreased locomotor activity / IMPC
abnormal femur morphology / IMPC
abnormal scapula morphology / IMPC
decreased body weight / IMPC
scoliosis / IMPC
increased neutrophil cell number / IMPC
abnormal spine curvature / IMPC
increased circulating potassium level / IMPC
decreased lymphocyte cell number / IMPC
abnormal humerus morphology / IMPC
abnormal lens morphology / IMPC
abnormal rib morphology / IMPC
increased circulating alkaline phosphatase level / IMPC
decreased exploration in new environment / IMPC
increased anxiety-related response / IMPC
abnormal pelvic girdle bone morphology / IMPC
abnormal fibula morphology / IMPC
increased freezing behavior / IMPC
abnormal radius morphology / IMPC
increased granulocyte number / IMPC
thrombocytosis / IMPC
decreased respiratory quotient / IMPC
enhanced cued conditioning behavior / IMPC
short tibia / IMPC
increased monocyte cell number / IMPC
increased leukocyte cell number / IMPC
decreased circulating glucose level / IMPC
decreased circulating serum albumin level / IMPC
abnormal mandible morphology / IMPC
abnormal gait / IMPC

MGI phenotypes (gene matching)

delayed bone ossification / MGI
fragile skeleton / MGI
abnormal scapula morphology / MGI
abnormal rib morphology / MGI
abnormal tibia morphology / MGI
abnormal femur morphology / MGI
decreased body height / MGI
decreased body size / MGI
abnormal gait / MGI
abnormal limb morphology / MGI
abnormal joint morphology / MGI
short femur / MGI
transmission ratio distortion / MGI
small scapula / MGI
short humerus / MGI
abnormal deltoid tuberosity morphology / MGI
abnormal ischium morphology / MGI
abnormal thoracic cage morphology / MGI
short ribs / MGI
small ischium / MGI
abnormal vertebral column morphology / MGI
abnormal humerus morphology / MGI
small cranium / MGI
skeleton phenotype / MGI
abnormal skeleton morphology / MGI
increased long bone epiphyseal plate size / MGI
increased diameter of femur / MGI
increased diameter of humerus / MGI
abnormal intramembranous bone ossification / MGI
abnormal calcaneum morphology / MGI
lethality, incomplete penetrance / MGI
absent bone trabeculae / MGI
increased alkaline phosphatase activity / MGI
decreased femur compact bone thickness / MGI
thin neurocranium / MGI

Literature references

Genome-wide, large-scale production of mutant mice by ENU mutagenesis.;Hrabé de Angelis M H, Flaswinkel H, Fuchs H, Rathkolb B, Soewarto D, Marschall S, Heffner S, Pargent W, Wuensch K, Jung M, Reis A, Richter T, Alessandrini F, Jakob T, Fuchs E, Kolb H, Kremmer E, Schaeble K, Rollinski B, Roscher A, Peters C, Meitinger T, Strom T, Steckler T, Holsboer F, Klopstock T, Gekeler F, Schindewolf C, Jung T, Avraham K, Behrendt H, Ring J, Zimmer A, Schughart K, Pfeffer K, Wolf E, Balling R, ;2000;Nature genetics;25;444-7; 10932192
Screening for dysmorphological abnormalities--a powerful tool to isolate new mouse mutants.;Fuchs H, Schughart K, Wolf E, Balling R, Hrabé de Angelis M, ;2000;Mammalian genome : official journal of the International Mammalian Genome Society;11;528-30; 10886017
Exome sequencing identifies a nonsense mutation in Fam46a associated with bone abnormalities in a new mouse model for skeletal dysplasia.;Diener Susanne, Bayer Sieglinde, Sabrautzki Sibylle, Wieland Thomas, Mentrup Birgit, Przemeck Gerhard K H, Rathkolb Birgit, Graf Elisabeth, Hans Wolfgang, Fuchs Helmut, Horsch Marion, Schwarzmayr Thomas, Wolf Eckhard, Klopocki Eva, Jakob Franz, Strom Tim M, Hrabě de Angelis Martin, Lorenz-Depiereux Bettina, ;2016;Mammalian genome : official journal of the International Mammalian Genome Society;27;111-21; 26803617

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C3HeB/FeJ-Tent5am1Mhda/Ieg