MGI phenotypes (allele matching)
B6.129P2-Sema4bGt(RST235)Byg/Ieg
| Status | Available to order |
| EMMA ID | EM:03097 |
| Citation information | RRID:IMSR_EM:03097 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2-Sema4bGt(RST235)Byg/Ieg |
| Alternative name | RST235 (Sema4b) |
| Strain type | Gene-trap |
| Allele/Transgene symbol | Sema4bGt(RST235)Byg |
| Gene/Transgene symbol | Sema4b |
Information from provider
| Provider | Roland Friedel |
| Provider affiliation | Soriano Lab, Department of Developomental & Regenerative Biology, Mount Sinai School of Medicine |
| Genetic information | Gene Trap in the last intron of Sema4b gene. The trap vector pGT1TMpfs contains a transmembrane domain, generating a fusion protein that is retained intracellularly, leading to a loss of function allele. The trap vector contains lacZ and PLAP reporters. |
| Phenotypic information | Homozygous mutants are viable and fertile. No obvious phenotype. |
| Breeding history | Backcrossed for 11 generations to C57BL/6. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous C57BL/6J males, wild-type C57BL/6J females |
| Stage of embryos | 2-cell |
Disease and phenotype information
Literature references
- Defining brain wiring patterns and mechanisms through gene trapping in mice.;Leighton P A, Mitchell K J, Goodrich L V, Lu X, Pinson K, Scherz P, Skarnes W C, Tessier-Lavigne M, ;2001;Nature;410;174-9; 11242070
- Functional analysis of secreted and transmembrane proteins critical to mouse development.;Mitchell K J, Pinson K I, Kelly O G, Brennan J, Zupicich J, Scherz P, Leighton P A, Goodrich L V, Lu X, Avery B J, Tate P, Dill K, Pangilinan E, Wakenight P, Tessier-Lavigne M, Skarnes W C, ;2001;Nature genetics;28;241-9; 11431694
- Semaphorin-Plexin Signaling Controls Mitotic Spindle Orientation during Epithelial Morphogenesis and Repair.;Xia Jingjing, Swiercz Jakub M, Bañón-Rodríguez Inmaculada, Matković Ivana, Federico Giuseppina, Sun Tianliang, Franz Timo, Brakebusch Cord H, Kumanogoh Atsushi, Friedel Roland H, Martín-Belmonte Fernando, Gröne Hermann-Josef, Offermanns Stefan, Worzfeld Thomas, ;2015;Developmental cell;33;299-313; 25892012