MGI phenotypes (allele matching)
B6;129P2-Plxnb1tm1Matl/Ieg
| Status | Available to order |
| EMMA ID | EM:03099 |
| Citation information | RRID:IMSR_EM:03099 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;129P2-Plxnb1tm1Matl/Ieg |
| Alternative name | Plexin-B1 KO |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Plxnb1tm1Matl |
| Gene/Transgene symbol | Plxnb1 |
Information from provider
| Provider | Roland Friedel |
| Provider affiliation | Soriano Lab, Department of Developomental & Regenerative Biology, Mount Sinai School of Medicine |
| Genetic information | Official allele name: Plxnb1tm1Matl. Knock-out by targeted trapping of the plexin B1 gene. Targeted mutagenesis using a promoterless gene trap vector ("targeted trapping") was used to insert a 9.4-kb placental alkaline phosphatase (PLAP) secretory cassette into intron 21. This results in transcription of a fusion mRNA joining upstream exons of the endogenous gene to the elements of the cassette, comprising a splice acceptor and a transmembrane domain coding sequence followed by the betageo (lacZ/neomycin resistance fusion) cDNA, an internal ribosomal entry site (IRES), and the human PLAP sequence with a polyadenylation signal. Two proteins are translated from this bicistronic transcript: a PLXNB1/Betageo fusion protein that is sequestered in an intracellular compartment of the neuronal cell body; and PLAP, which is expressed on the axonal surface. |
| Phenotypic information | Homozygous viable and fertile. Plxnb1-/- embryonic kidneys are larger in size and have increased ureteric branching. Plexin B1/plexin B2 double mutants are early embryonic lethal. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous C57BL/6J males, wild-type C57BL/6J females |
| Stage of embryos | 2-cell |
Disease and phenotype information
Literature references
- Gene targeting using a promoterless gene trap vector ("targeted trapping") is an efficient method to mutate a large fraction of genes.;Friedel Roland H, Plump Andrew, Lu Xiaowei, Spilker Kerri, Jolicoeur Christine, Wong Karen, Venkatesh Tadmiri R, Yaron Avraham, Hynes Mary, Chen Bin, Okada Ami, McConnell Susan K, Rayburn Helen, Tessier-Lavigne Marc, ;2005;Proceedings of the National Academy of Sciences of the United States of America;102;13188-93; 16129827
- Plexin-B2, but not Plexin-B1, critically modulates neuronal migration and patterning of the developing nervous system in vivo.;Deng Suhua, Hirschberg Alexandra, Worzfeld Thomas, Penachioni Junia Y, Korostylev Alexander, Swiercz Jakub M, Vodrazka Peter, Mauti Olivier, Stoeckli Esther T, Tamagnone Luca, Offermanns Stefan, Kuner Rohini, ;2007;The Journal of neuroscience : the official journal of the Society for Neuroscience;27;6333-47; 17554007
- A functional role for semaphorin 4D/plexin B1 interactions in epithelial branching morphogenesis during organogenesis.;Korostylev Alexander, Worzfeld Thomas, Deng Suhua, Friedel Roland H, Swiercz Jakub M, Vodrazka Peter, Maier Viola, Hirschberg Alexandra, Ohoka Yoshiharu, Inagaki Shinobu, Offermanns Stefan, Kuner Rohini, ;2008;Development (Cambridge, England);135;3333-43; 18799546