| Status | Available to order |
| EMMA ID | EM:03102 |
| Citation information | RRID:IMSR_EM:03102 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | Cnbc:NMRI-Tg(Tyr*)2138Lmon |
| Alternative name | YRT4 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Tyr*)2138Lmon, Tyrc |
| Gene/Transgene symbol | Tg(Tyr*)2138Lmon, Tyr |
| Provider | Lluis Montoliu |
| Provider affiliation | Departamento de Biologia Molecular y Celular, CNB-CSIC, Centro Nacional de Biotecnologia |
| Additional owner | Prof. Dr. Günther Schütz, DKFZ, Dept. Molecular Biology of the Cell I, Heidelberg, Germany |
| Genetic information | Transgenic mice carrying 1 copy of the 100 kb YAC YRT4 containing the entire mouse tyrosinase gene, including all five exons, 5' UTR and 3' UTR without the LCR regulatory element. YRT4 YAC was obtained after removing 150 kb of DNA including LCR and upstream sequences, by homologous recombination of YRT2 YAC (Montoliu et al. 1996; EMBO J 15:6026-34). Mice were generated in the albino outbred WinHsd:NMRI background whose albino phenotype was partially rescued by the YAC YRT4 transgene, thereby becoming light pigmented (light grey, patchy, coat colour variegation) and eye pigmented. The YAC YRT4 is derived from a YAC library made from C3H mouse genomic DNA. |
| Phenotypic information | Lightly pigmented, variegated, black eyes. Transgenic mice YRT4 in the NMRI background have pigmented eyes and very light skin pigmentation, variegated coat colour, with patches of pigmented skin and hair. They have partially rescued the retinal and visual abnormalities associated with albinism. In particular, they have variegation in retinal pigment epithelium cells with a peculiar distribution of pigmentation, resulting in peripheral retina being mostly pigmented and central retina being mostly albino. |
| Breeding history | Transgenic mice YRT4 were generated in 1994/95 by pronuclear microinjection using fertilised albino outbred NMRI oocytes and have been maintained as hemizygous since then in the same background. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous NMRI males, wild-type NMRI females |
| Stage of embryos | 8-cell |
Orphanet associated rare diseases, based on orthologous gene matching
